Determining feline proBNP

• US 9,605,068 B2
• Filed: 02/27/2009
• Issued: 03/28/2017
• Est. Priority Date: 09/08/2004
• Status: Active Grant

• Current Assignee: The Antibody Lab GmbH
• Original Assignee: The Antibody Lab GmbH
• Inventors: Woloszczuk, Wolfgang, Hawa, Gerhard
• Primary Examiner(s): FOSTER, CHRISTINE E
• Application Number:
  US12/394,592
• Publication Number:
  US 20090170135A1
• Time in Patent Office: 2,951 Days
• US Class Current: 1/1
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: G01N 2333/58 Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
• CPC Class Codes: G01N 2800/32 Cardiovascular disorders
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

The invention relates to a method of determining feline or canine proBNP or fragments thereof for the diagnosis of heart disease, comprising the steps of providing a feline or canine sample, contacting the sample with at least one species-specific antibody for binding to at least one epitope of the respective species proBNP, determining the concentration of at least one fragment of proBNP, and diagnosing the animal with heart disease if the concentration is elevated compared to healthy subjects.

Claims

1. A method for detecting feline NT-proBNP comprising:

obtaining a first polyclonal antibody raised against a peptide consisting of the sequence HPLGGPGPASEASAIQELLD (SEQ ID NO;

6);

obtaining a second polyclonal antibody raised against a peptide consisting of the sequence VLAPHDNVLRALRRLGSSKM (SEQ ID NO;

9);

contacting said first and second antibodies for binding feline NT-proBNP with a blood sample from a feline subject; and

measuring the concentration of feline NT-proBNP in said blood sample by detecting the binding of said first and second antibodies to feline NT-proBNP.

2. The method of claim 1, wherein at least one of said first antibody and said second antibody is bound to a solid phase.

3. The method of claim 1, wherein the blood sample is serum.