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Methods and products for transfecting cells

  • US 9,605,277 B2
  • Filed: 07/11/2016
  • Issued: 03/28/2017
  • Est. Priority Date: 12/05/2011
  • Status: Active Grant
First Claim
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1. A method for treating HIV infection in a human subject, comprising:

  • (a) harvesting a hematopoietic cell from the subject;

    (b) gene-editing the hematopoietic cell by transfecting the hematopoietic cell with an in vitro transcribed synthetic RNA molecule encoding a gene-editing protein, whereinthe hematopoietic cell expresses the gene-editing protein, andthe gene-editing protein comprises a DNA-binding domain and a nuclease catalytic domain that causes a double-strand break in the DNA of the hematopoietic cell to reduce the function of a gene selected from CCR5 and CXCR4; and

    (c) administering the gene-edited hematopoietic cell to the subject to result in the treatment of HIV infection in the subject.

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