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Nucleic acid sequencing using tags

  • US 9,605,309 B2
  • Filed: 11/06/2013
  • Issued: 03/28/2017
  • Est. Priority Date: 11/09/2012
  • Status: Active Grant
First Claim
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1. A method for sequencing a nucleic acid sample with the aid of a nanopore in a membrane adjacent to a sensing electrode, the method comprising:

  • (a) providing tagged nucleotides into a reaction chamber comprising said nanopore, wherein an individual tagged nucleotide of said tagged nucleotides contains a tag coupled to a nucleotide, which tag is detectable with the aid of said nanopore;

    (b) carrying out a polymerization reaction with the aid of a single polymerase coupled to the nanopore, thereby incorporating an individual tagged nucleotide of said tagged nucleotides into a growing strand complementary to a single stranded nucleic acid molecule from said nucleic acid sample; and

    (c) detecting, with the aid of said nanopore, a tag associated with said individual tagged nucleotide during incorporation of said individual tagged nucleotide, wherein said tag is detected a plurality of times with the aid of said nanopore while said nucleotide is associated with said polymerase and said electrode is re-charged between tag detection periods.

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