Systems and devices for profiling microbiota of skin

• US 9,610,037 B2
• Filed: 11/27/2013
• Issued: 04/04/2017
• Est. Priority Date: 11/27/2013
• Status: Active Grant

• Current Assignee: Elwha LLC (Intellectual Ventures LLC)
• Original Assignee: Elwha LLC (Intellectual Ventures LLC)
• Inventors: Baym, Michael H., Hyde, Roderick A., Kare, Jordin T., Leuthardt, Eric C., McKnight, Gary L., Pan, Tony S., Sweeney, Elizabeth A., Tegreene, Clarence T., Wood, Jr., Lowell L.
• Primary Examiner(s): White, Dennis M
• Assistant Examiner(s): KILPATRICK, BRYAN T
• Application Number:
  US14/091,805
• Publication Number:
  US 20150148265A1
• Time in Patent Office: 1,224 Days
• CPC Class Codes: A61B 10/02 Instruments for taking cell samples or for biopsy A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48
• CPC Class Codes: A61B 2010/0225 for taking multiple samples
• CPC Class Codes: A61B 2090/3941 Photoluminescent markers
• CPC Class Codes: A61B 2090/3975 active
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: A61B 5/0077 Devices for viewing the surface of the body, e.g. camera, magnifying lens
• CPC Class Codes: A61B 5/0095 by applying light and detecting acoustic waves, i.e. photoacoustic measurements
• CPC Class Codes: A61B 5/443 Evaluating skin constituents, e.g. elastin, melanin, water
• CPC Class Codes: A61B 5/445 Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore
• CPC Class Codes: A61B 5/4839 combined with drug delivery
• CPC Class Codes: A61B 5/486 Bio-feedback A61B5/375 takes precedence
• CPC Class Codes: A61B 5/7246 using correlation, e.g. template matching or determination of similarity
• CPC Class Codes: A61B 90/98 using electromagnetic means, e.g. transponders
• CPC Class Codes: G01N 2800/20 Dermatological disorders
• CPC Class Codes: G01N 33/56911 Bacteria
• CPC Class Codes: G01N 33/56938 Staphylococcus

Abstract

Devices and systems for sampling and profiling microbiota of skin are described which include a replaceable microbe sampling unit including a substrate and a location information storage component, the substrate including a microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual, and the location information storage component configured to store information associated with the location of said one or more regions of the skin surface of the individual.

Claims

1. A microbe profiling system comprising:

a replaceable microbe sampling unit including

a substrate including a microbe-capture region, the microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual; and

a location information storage component configured to store information associated with the location of said one or more regions of the skin surface of the individual;

a microbe sampling device including

a device head including an epidermis-engaging component and at least one access opening, the epidermis-engaging component configured to dislodge at least one type of microbe from the skin surface of the individual; and

a hand-held housing, at least a portion of the hand-held housing defining an opening aligned with the at least one access opening of the device head and including

a region sized for receiving the replaceable microbe sampling unit, the region configured to position at least a portion of the substrate of the replaceable microbe sampling unit in operable communication with the opening defined by the hand-held housing;

at least one motivatable component configured to move at least a portion of the substrate of the replaceable microbe sampling unit;

a motor operably coupled to the at least one motivatable component, the motor including circuitry to drive the at least one motivatable component; and

a location-capture component including circuitry to determine the location of one or more regions of the skin surface of the individual as the epidermis-engaging component contacts said one or more regions of the skin surface of the individual and to output information associated with the location of said one or more regions of the skin surface to the location information storage component of the replaceable microbe sampling unit; and

an analyzer including

a receiving region sized to accept the replaceable microbe sampling unit;

at least one sensor component including circuitry configured to detect one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit and to transform the detected one or more signals into a sensor output including information associated with at least one property of the detected one or more signals; and

at least one location information reader including circuitry configured to read the information associated with the location of said one or more regions of the skin surface of the individual from the location information storage component of the replaceable microbe sampling unit and to transform the information into a location output.

2. The system of claim 1, wherein the substrate of the replaceable microbe sampling unit comprises at least one of a disc shape, an elongated flexible strip, or a sheet.

3. The system of claim 1, wherein the microbe-capture region comprises at least one of a charged surface, an adhesive, a biomolecule-binding polymer, or a gel.

4. The system of claim 1, wherein the microbe-capture region comprises a plurality of specific microbe-binding elements.

5. The system of claim 1, wherein the microbe-capture region comprises a plurality of signal-generating complexes.

6. The system of claim 5, wherein each of the plurality of signal-generating complexes comprises at least one signal-generating element operably coupled to at least one specific microbe-binding element, the at least one signal-generating element configured to emit one or more signals in response to contact with at least one type of microbe by the operably coupled at least one specific microbe-binding element.

7. The system of claim 1, wherein the location information storage component of the replaceable microbe sampling unit comprises an electronic location information storage component.

8. The system of claim 1, wherein the location information storage component of the replaceable microbe sampling unit comprises computer readable media.

9. The system of claim 1, wherein the location information storage component of the replaceable microbe sampling unit includes a transmitter with circuitry configured to transmit the information associated with the location of said one or more regions of the skin surface of the individual.

10. The system of claim 1, wherein the location information storage component of the replaceable microbe sampling unit is part of the substrate.

11. The system of claim 1, wherein the location information storage component of the replaceable microbe sampling unit comprises at least one mark on the substrate, the at least one mark including information associated with the location of said one or more regions of the skin surface of the individual.

12. The system of claim 1, wherein the replaceable microbe sampling unit comprises a cassette including the substrate and the location information storage component.

13. The system of claim 12, wherein the cassette includes at least one motivatable component.

14. The system of claim 1, wherein the at least one sensor component of the analyzer includes circuitry configured to detect one of more signals emitted or reflected from at least one of at least one type of microbe captured on the microbe-capture region of the replaceable microbe sampling unit or at least one of a plurality of signal-generating elements associated with at least one type of microbe captured on the microbe-capture region of the replaceable microbe sampling unit.

15. The system of claim 1, wherein the at least one sensor component of the analyzer includes circuitry configured to detect one or more signals emitted from at least one of a plurality of signal-generating complexes associated with the substrate of the replaceable microbe sampling unit in response to contact with at least one type of microbe.

16. The system of claim 1, wherein the analyzer includes a plurality of at least one type of signal-generating element.

17. The system of claim 1, wherein the at least one location information reader of the analyzer includes at least one of an optical reader, an RFID tag reader, an electronic reader, or a magnetic reader.

18. The system of claim 1, wherein the analyzer further comprises a motor operably coupled to at least one motivatable component, the at least one motivatable component configured to move the substrate of the replaceable microbe sampling unit.

19. The system of claim 1, further comprising

a computing device including a processor, the computing device operably coupled to the analyzer, and including circuitry to

receive the location output from the analyzer, the location output including information associated with the location of said one or more regions of the skin surface of the individual;

receive the sensor output from the analyzer, the sensor output including the information associated with the at least one property of the detected one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit;

compare the at least one property of the one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit with a database of reference signal properties;

generate an alignment of the location of said one or more regions of the skin surface of the individual with the detected one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit;

generate a microbe profile based on the alignment, the microbe profile including a spatial distribution of at least one type of microbe on the skin surface of the individual; and

report the microbe profile to a user.

20. The system of claim 19, wherein the computing device includes circuitry configured to

identify at least one type of microbe captured on the microbe-capture region from said one or more regions of the skin surface of the individual based on the comparison of the at least one property of the detected one or more signals from the at least one type of microbe with the database of reference microbe signal properties;

and

report to the user an identity of the at least one type of microbe.

21. The system of claim 19, wherein the computing device includes circuitry configured to

generate a recommended treatment regimen based on at least one of the spatial distribution or an identity of the at least one type of microbe on the skin surface of the individual;

and

report to the user the recommended treatment regimen.

22. The system of claim 19, wherein the computing device and the analyzer are part of a kiosk.

23. The system of claim 1, wherein the microbe sampling device further comprises a marking means including circuitry configured to receive the location information from the location-capture component and to deposit at least one mark on the substrate of the replaceable microbe sampling unit, the at least one mark representative of the information associated with the location of one or more regions on the skin surface of the individual.

35. The system of claim 5, wherein the microbe-capture region comprises a plurality of signal-generating complexes of at least one first type able to emit at least one first signal type in response to at least one first type of microbe and a plurality of signal-generating complexes of at least one second type able to emit at least one second signal type in response to at least one second type of microbe.

36. The system of claim 11, wherein the at least one mark includes at least one of a chromophore, a fluorophore, a bar code, an electrical charge, a magnetic substance, a quantum dot, or a radiofrequency identification tag.

24. A microbe profiling system comprising:

a replaceable microbe sampling unit including

a substrate including a microbe-capture region; and

a location information storage component;

a microbe sampling device including

a device head including an epidermis-engaging component and at least one access window, the device head configured to dislodge at least one type of microbe from a skin surface of the individual; and

a hand-held housing, at least a portion of the hand-held housing defining an opening aligned with the at least one access window of the device head, the hand-held housing including

a region sized for receiving the replaceable microbe sampling unit, the region configured to position at least a portion of the substrate of the replaceable microbe sampling unit in operable communication with the opening defined by the hand-held housing;

at least one motivatable component operably coupled to the substrate of the replaceable microbe sampling unit;

a motor operably coupled to the at least one motivatable component, the motor including circuitry to drive the at least one motivatable component; and

a location-capture component including circuitry to determine a location of one or more regions of the skin surface of the individual as the epidermis-engaging component contacts said one or more regions of the skin surface of the individual and to output information associated with the location of said one or more regions of the skin surface of the individual to the location information storage component of the replaceable microbe sampling unit;

an analyzer including

a receiving region sized to accept the replaceable microbe sampling unit;

at least one sensor component including circuitry configured to detect one or more signals emitted or reflected from the microbe-capture region of the substrate of the replaceable microbe sampling unit and to transform the detected one or more signals into a sensor output including information associated with at least one property of the detected one or more signals; and

at least one location information reader including circuitry to read the information associated with the location of said one or more regions of the skin surface of the individual from the location information storage component of the replaceable microbe sampling unit and to transform the information into a location output; and

a computing device including a processor, the computing device including circuitry configured to

receive the location output from the analyzer, the location output including information associated with the location of said one or more regions of the skin surface of the individual;

receive the sensor output from the analyzer, the sensor output including the information associated with the at least one property of the detected one or more signals emitted or reflected from the microbe-capture region;

compare the at least one property of the one or more signals emitted or reflected from the microbe-capture region with a database of reference signal properties;

generate an alignment of the location of said one or more regions of the skin surface of the individual with the detected one or more signals emitted or reflected from the microbe-capture region;

generate a microbe profile based on the alignment, the microbe profile including a spatial distribution of at least one type of microbe on the skin surface of the individual; and

report the microbe profile to a user.

25. A microbe profiling system comprising:

a replaceable microbe sampling unit including

at least one motivatable component;

a substrate disposed in relation to the at least one motivatable component, the substrate including a microbe-capture region; and

a location information storage component;

a microbe sampling device including

a device head including an epidermis-engaging component and at least one access window, the device head configured to dislodge at least one type of microbe from the skin surface of the individual; and

a hand-held housing, at least a portion of the hand-held housing defining an opening aligned with the at least one access window of the device head, the hand-held housing including

a region sized for receiving the replaceable microbe sampling unit, the region configured to position at least a portion of the substrate of the replaceable microbe sampling unit in operable communication with the opening defined by the hand-held housing;

a motor operably coupled to the at least one motivatable component of the replaceable microbe sampling unit, the motor including circuitry to drive the at least one motivatable component; and

a location-capture component including circuitry to determine a location of one or more regions of the skin surface of the individual as the epidermis-engaging component contacts said one or more regions of the skin surface of the individual and to output information associated with the location of the one or more regions of the skin surface of the individual to the location information storage component of the replaceable microbe sampling unit;

an analyzer including

a receiving region sized to accept the replaceable microbe sampling unit;

at least one sensor component including circuitry configured to detect one or more signals emitted or reflected from the microbe-capture region of the substrate of the replaceable microbe sampling unit and to transform the detected one or more signals into a sensor output including information associated with at least one property of the detected one or more signals; and

at least one location information reader including circuitry to read the information associated with the location of the one or more regions of the skin surface of the individual from the location information storage component of the replaceable microbe sampling unit and to transform the information into a location output; and

a computing device including a processor, the computing device including circuitry configured to

receive the location output from the analyzer, the location output including information associated with the location of said one or more regions of the skin surface of the individual;

receive the sensor output from the analyzer, the sensor output including the information associated with the at least one property of the detected one or more signals emitted or reflected from the microbe-capture region;

compare the at least one property of the one or more signals emitted or reflected from the microbe-capture region with a database of reference signal properties;

generate an alignment of the location of said one or more regions of the skin surface of the individual with the detected one or more signals emitted or reflected from the microbe-capture region;

generate a microbe profile based on the alignment, the microbe profile including a spatial distribution of at least one type of microbe on the skin surface of the individual; and

report the microbe profile to a user.

26. A microbe sampling unit comprising:

a substrate including a microbe-capture region, the microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual; and

a location information storage component configured to store information associated with the location of the one or more regions of the skin surface of the individual.

27. The device of claim 26, further comprising a housing, at least a portion of the housing defining an opening, the substrate and the location information storage component at least partially contained within the housing, the substrate at least partially disposed in the opening defined by the housing.

28. The device of claim 27, wherein the housing includes at least one motivatable component, the substrate disposed on a surface of the at least one motivatable component.

29. The device of claim 26, wherein the microbe-capture region comprises a plurality of specific-microbe binding elements.

30. The device of claim 26, wherein the location information storage component comprises a memory card.

31. A microbe profiling system comprising:

an analyzer including

a receiving region sized to accept a replaceable microbe sampling unit, the replaceable microbe sampling unit including a substrate with a microbe-capture region to capture at least one type of microbe from one or more regions of a skin surface of an individual and a location information storage component including information associated with a location of said one or more regions of the skin surface of the individual;

at least one sensor component including circuitry configured to detect one or more signals emitted or reflected from the microbe-capture region of the substrate of the replaceable microbe sampling unit and to transform the detected one or more signals into a sensor output including information associated with at least one property of the detected one or more signals; and

at least one location information reader including circuitry to read the information associated with the location of the one or more regions of the skin surface of the individual from the location information storage component of the replaceable microbe sampling unit and to transform the information into a location output;

a computing device including a processor, the computing device operably coupled to the analyzer; and

non-transitory machine readable media bearing one or more instructions for generating a microbe profile, the one or more instructions including

one or more instructions for receiving the location output from the analyzer, the location output including information associated with the location of the one or more regions of the skin surface of the individual;

one or more instructions for receiving the sensor output from an analyzer, the sensor output including information associated with the at least one property of the one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit;

one or more instructions for comparing the at least one property of the one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit with a database of reference signal properties;

one or more instructions for identifying the at least one type of microbe captured on the microbe-capture region of the replaceable microbe sampling unit based on the comparison of the at least one property of the one or more signals emitted or reflected from the microbe-capture region of the replaceable microbe sampling unit with the database of reference signal properties;

one or more instructions for generating an alignment of the location of said one or more regions of the skin surface of the individual with the one or more signals emitted or reflected from the microbe-capture region;

one or more instructions for generating the microbe profile based on the alignment, the microbe profile including at least one of an identity or a spatial distribution of the at least one type of microbe on the skin surface of the individual; and

one or more instructions for reporting the microbe profile to a user.

32. The system of claim 31, wherein the one or more instructions include one or more instructions for generating a recommended treatment regimen based on a comparison of the microbe profile with a reference microbe profile;

and one or more instructions for reporting the recommended treatment regimen to the user.

33. The system of claim 31, further comprising:

a replaceable microbe sampling unit.

34. The system of claim 31, further comprising:

a microbe sampling unit.