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Multi-layer tissue systems and methods

  • US 9,616,152 B2
  • Filed: 05/15/2013
  • Issued: 04/11/2017
  • Est. Priority Date: 04/25/2008
  • Status: Active Grant
First Claim
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1. A method of manufacturing a multi-layer amnion tissue patch, the method comprising:

  • contacting a first fetal sac amnion tissue layer having an epithelial layer, a basement membrane, a compact layer, and a fibroblast layer from which a spongy layer has been removed, the fibroblast layer including a loose network of reticulum, with a second fetal sac amnion tissue layer having an epithelial layer, a basement membrane, a compact layer, and a fibroblast layer from which a spongy layer has been removed, the fibroblast layer including a loose network of reticulum;

    placing the contacted first and second fetal sac amnion tissue layers in a pouch;

    removing air from the pouch while keeping the contacted first and second fetal sac amnion tissue layers moist;

    placing the pouch containing the contacted first and second fetal sac amnion tissue layers in a press mechanism; and

    pressing the pouch with the press mechanism, so that the first amnion tissue layer and the second amnion tissue layer are pressed against each other so as to form the multi-layer amnion tissue patch, such that the epithelial layers of the first and second tissue layers define first and second opposing outer surfaces of the patch, respectively, and a compressed, coupled fibroblast region is disposed between the first and second opposing outer surfaces of the patch, the compressed, coupled fibroblast region having an interface of entangled fibers of the first and second fibroblast layers.

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