Tissue-specific differentiation matrices and uses thereof
First Claim
1. A tissue-specific differentiation system comprisinga differentiation-inducing extracellular matrix generated ex vivo on a surface by culturing fibroblast cells on said surface, wherein the differentiation-inducing extracellular matrix is free of the fibroblast cells;
- anddifferentiated cells in contact with the differentiation-inducing extracellular matrix, wherein the differentiated cells are derived from isolated stem cells seeded onto the differentiation-inducing extracellular matrix and induced to undergo differentiation into differentiated cells of the same tissue type as the fibroblast cells b cultured on the differentiation-inducing extracellular extracellular matrix;
wherein the fibroblast cells and differentiated cells are both from one of the following tissue types;
neural tissue, epidermal tissue, dermal tissue, adipose tissue, cardiac tissue, kidney tissue, muscle tissue, liver tissue, cartilage tissue, pancreas tissue, tissue of the endometrium of uterus, umbilical cord tissue, or dental pulp tissue.
2 Assignments
0 Petitions
Accused Products
Abstract
In some aspects, this invention provides a method of making a bone marrow-derived tissue-specific stem cell proliferation, expansion, isolation and rejuvenation extracellular matrix. In other aspects, this invention provides a method of making a tissue-specific fibroblast-derived stem cell differentiation extracellular matrix. Also provided are methods of using such a cell-derived preservation or differentiation matrices to induce tissue-specific differentiation of pluripotent cells, repair damaged tissue, and treat a subject having a physiologic deficiency using the same.
-
Citations
37 Claims
-
1. A tissue-specific differentiation system comprising
a differentiation-inducing extracellular matrix generated ex vivo on a surface by culturing fibroblast cells on said surface, wherein the differentiation-inducing extracellular matrix is free of the fibroblast cells; - and
differentiated cells in contact with the differentiation-inducing extracellular matrix, wherein the differentiated cells are derived from isolated stem cells seeded onto the differentiation-inducing extracellular matrix and induced to undergo differentiation into differentiated cells of the same tissue type as the fibroblast cells b cultured on the differentiation-inducing extracellular extracellular matrix; wherein the fibroblast cells and differentiated cells are both from one of the following tissue types;
neural tissue, epidermal tissue, dermal tissue, adipose tissue, cardiac tissue, kidney tissue, muscle tissue, liver tissue, cartilage tissue, pancreas tissue, tissue of the endometrium of uterus, umbilical cord tissue, or dental pulp tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- and
-
9. A method of making a tissue-specific differentiation system comprising:
-
a) culturing fibroblast cells of a given tissue type ex vivo on a surface to produce a differentiation-inducing extracellular matrix; b) removing the fibroblast cells from the differentiation-inducing extracellular matrix; c) seeding isolated stem cells on the differentiation-inducing extracellular matrix; and d) culturing the isolated stem cells on the differentiation-inducing extracellular matrix, thereby inducing the stem cells to differentiate into differentiated cells of the same tissue type as the fibroblast cells cultured in step (a); wherein the fibroblast cells cultured in step and the differentiated cells produced in step (d) are both from one of the following tissue types;
neural tissue, epidermal tissue, dermal tissue, adipose tissue, cardiac tissue, kidney tissue, muscle tissue, liver tissue, cartilage tissue, pancreas tissue, tissue of the endometrium of uterus, umbilical cord tissue, or dental pulp tissue. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
-
23. A method of inducing tissue-specific differentiation of isolated stem cells into a differentiated cell type comprising:
-
a) generating a first differentiation factor comprising a differentiation-inducing extracellular matrix by culturing fibroblast cells on a surface; and b) contacting the isolated stem cells with the first differentiation factor, thereby inducing the stem cells to differentiate into the differentiated cell type, wherein the differentiated cell type is of the same tissue type as the fibroblast cells, wherein the fibroblast cells have been removed from the differentiation-inducing extracellular matrix before contacting the isolated stem cells with the differentiation-inducing extracellular matrix, and wherein the fibroblast cells and differentiated cells are both from one of the following tissue types;
neural tissue, epidermal tissue, dermal tissue, adipose tissue, cardiac tissue, kidney tissue, muscle tissue, liver tissue, cartilage tissue, pancreas tissue, tissue of the endometrium of uterus, umbilical cord tissue, or dental pulp tissue. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
-
Specification