Muscle-derived cells (MDCs) for promoting and enhancing nerve repair and regeneration

• US 9,617,516 B2
• Filed: 04/26/2004
• Issued: 04/11/2017
• Est. Priority Date: 04/25/2003
• Status: Active Grant

• Current Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Original Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Inventors: Minnery, Brandon, Capelli, Chistopher C., Chancellor, Michael B., Huard, Johnny
• Primary Examiner(s): WANG, CHANG YU
• Application Number:
  US10/832,542
• Publication Number:
  US 20050238625A1
• Time in Patent Office: 4,733 Days
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/34 Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes vascular smooth muscle A61K35/44
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/02 for peripheral neuropathies
• CPC Class Codes: C12N 5/0658 Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts

Abstract

The present invention describes methods involving the use of muscle derived cells (MDCs), preferably obtained from skeletal muscle, to support the innervation and repair of damaged tissues and organs, particularly associated with nerve damage or neuropathy. The invention relates to MDCs for use in methods for promoting or enhancing innervation of nerve cells, particularly in the peripheral nervous system, and their ability to contribute to the development of neuronal tissue when MDCs are introduced at or near a tissue or organ site in need of repair due to injury, damage, disease, or dysfunction. Such methods are useful for the treatment of central and peripheral nervous system disorders and to alleviate, abate, or eliminate the symptoms of neurologic or neurodegenerative diseases in animals, particularly mammals, including humans. The methods are also useful for treating both nerve and muscle tissue following injury, damage, or dysfunction to these tissue types.

Claims

1. A method of protecting penile nerve during recovery after a radical prostatectomy anastomosis in a human subject in need thereof, comprising:

injecting a cell suspension of isolated autologous adult human muscle-derived cells (MDCs) into said subject at the urethra or periurethral tissue at the time of the radical prostatectomy anastomosis, wherein said isolated MDCs are injected in an amount effective to protect the penile nerve, wherein the protection of the nerve during recovery treats erectile dysfunction and/or urinary incontinence;

wherein the MDCs are enriched from a muscle cell suspension by plating the suspension into at least two successive cultures so as to eliminate fibroblasts and non-muscle cells and to enrich for an end population of mononucleated MDCs, wherein the enriched end population of mononucleated MDCs comprises myoblasts;

wherein the MDCs are not genetically engineered to express heterologous genes and wherein the MDCs are introduced in an amount of about 105 to 106 cells per cm3 of tissue or organ in a physiologically acceptable medium.

2. The method according to claim 1, wherein the MDCs are obtained from skeletal muscle tissue.

3. The method according to claim 1, wherein a cloned population of the MDCs is introduced into the human subject.