Methods for maturing cardiomyocytes and uses thereof
First Claim
1. A method for inducing maturation of a cultured cardiomyocyte comprising contacting in vitro a cultured cardiomyocyte derived from a pluripotent stem cell or an induced pluripotent stem cell (iPS cell) with an isolated microRNA from the let-7 family, or with a vector that expresses said miRNA of the let-7 family, thereby inducing maturation of the cultured cardiomyocyte.
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Abstract
Described herein are methods and compositions useful for inducing maturation of a cardiomyocyte to a mature (e.g., adult) phenotype, such that the function and morphology of the mature cardiomyocyte matches or more closely mimics that of the adult heart. The methods and compositions use Let-7 miRNAs and modified forms thereof. Such methods and compositions permit the study and treatment of adult-onset cardiac diseases, disorders or injuries with mature cardiomyocytes that mimic the heart function of an adult. Methods of using cardiomyocytes matured in this manner for drug identification and drug cardiotoxicity testing are also provided.
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Citations
9 Claims
- 1. A method for inducing maturation of a cultured cardiomyocyte comprising contacting in vitro a cultured cardiomyocyte derived from a pluripotent stem cell or an induced pluripotent stem cell (iPS cell) with an isolated microRNA from the let-7 family, or with a vector that expresses said miRNA of the let-7 family, thereby inducing maturation of the cultured cardiomyocyte.
Specification