Manufacturing method for sugar solution and device for same

• US 9,624,516 B2
• Filed: 03/14/2011
• Issued: 04/18/2017
• Est. Priority Date: 03/15/2010
• Status: Active Grant

• Current Assignee: Toray Industries Incorporated
• Original Assignee: Toray Industries Incorporated
• Inventors: Kurihara, Hiroyuki, Minamino, Atsushi, Yamamoto, Yuki, Yamada, Katsushige
• Primary Examiner(s): Gitomer, Ralph
• Assistant Examiner(s): CLARKE, TRENT R
• Application Number:
  US13/634,961
• Publication Number:
  US 20130203117A1
• Time in Patent Office: 2,227 Days
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes
• CPC Class Codes: C12M 47/10 Separation or concentration of fermentation products bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02
• CPC Class Codes: C12P 19/00 Preparation of compounds containing saccharide radicals ketoaldonic acids C12P7/58
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.

Claims

1. A method of producing a sugar liquid by repeating a sugar liquid production process comprising (1) to (3):

(1) adding a filamentous fungus-derived cellulase to cellulose which is a processed product obtained by subjecting a cellulose-containing biomass to alkaline treatment, hydrothermal treatment or dilute sulfuric acid treatment and has not been subjected to enzyme treatment to perform primary hydrolysis;

(2) adding a fresh filamentous fungus-derived cellulase without adding cellulose to a hydrolysate formed in (1) without separating a solid residue in hydrolysate to perform secondary hydrolysis; and

(3) subjecting another hydrolysate formed in (2) to solid-liquid separation to obtain a sugar liquid and recovering enzyme from the sugar liquid;

wherein recovered enzyme obtained in (3) is used for (1) of next and later sugar liquid production processes, and

as said filamentous fungus-derived cellulase in (1) of the sugar liquid production process, an enzyme component recovered from a cellulose hydrolysate produced by a filamentous fungus-derived cellulase is used.

2. The method according to claim 1, wherein said filamentous fungus-derived cellulase in (1) or (2) comprises a component derived from a culture liquid of a microorganism belonging to the genus Trichoderma.

3. The method according to claim 1, wherein said recovered enzyme comprises xylanase and/or xylosidase.

4. The method according to claim 1, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

5. The method according to claim 1, wherein amounts of enzyme added in said primary hydrolysis and said secondary hydrolysis satisfy a following relation:

an amount of said recovered enzyme added in (1) >

an amount of said fresh enzyme added in (2).

6. The method according to claim 1, wherein recovery of said filamentous fungus-derived cellulase in (3) is carried out by filtering said sugar liquid through an ultrafiltration membrane and recovering said cellulase from the feed side.

7. The method according to claim 2, wherein said recovered enzyme comprises xylanase and/or xylosidase.

8. The method according to claim 2, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

9. The method according to claim 3, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

10. The method according to claim 1, wherein a reaction time of the primary hydrolysis in (1) is 15 minutes to 6 hours.

11. The method according to claim 10, wherein a reaction time of the secondary hydrolysis in (2) is 6 hours to 72 hours.