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Nanogrid rolling circle DNA sequencing

  • US 9,624,538 B2
  • Filed: 05/14/2008
  • Issued: 04/18/2017
  • Est. Priority Date: 11/14/2005
  • Status: Active Grant
First Claim
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1. A method of sequencing a polynucleotide immobilized on an array comprising:

  • synthesizing a concatemer of a polynucleotide by rolling circle amplification;

    immobilizing the concatemer to the array, wherein the array comprises a substrate having a plurality of specific functionalized regions attached to the substrate in a pattern forming an ordered array, wherein each specific functionalized region comprises an inner spot functionalized with a first group which serves to capture the concatemer and an outer spot functionalized with a second group which serves as an attachment point for an amplification primer, wherein the concatermer is immobilized to the inner spot, and wherein the concatemer has a cross-sectional diameter greater than the diameter of the inner spot; and

    sequencing the immobilized concatemer.

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