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DNA sequencing by synthesis using Raman and infrared spectroscopy detection

  • US 9,624,539 B2
  • Filed: 05/23/2012
  • Issued: 04/18/2017
  • Est. Priority Date: 05/23/2011
  • Status: Active Grant
First Claim
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1. A method for determining the sequence of consecutive nucleotide residues present in a single-stranded DNA comprising:

  • (a) contacting the single-stranded DNA, having a primer hybridized to a portion thereof, with a DNA polymerase and four different nucleoside triphosphate (NTP) analogues under conditions permitting the DNA polymerase to catalyze incorporation onto the primer of a NTP analogue complementary to a nucleotide residue of the single-stranded DNA which is immediately 5′

    to a nucleotide residue of the single-stranded DNA hybridized to the 3′

    terminal nucleotide residue of the primer, so as to form a DNA extension product, wherein (i) each of the four NTP analogues has the structure;

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