Extraction of materials from regions of interest in a sample

• US 9,625,355 B2
• Filed: 12/01/2014
• Issued: 04/18/2017
• Est. Priority Date: 12/01/2014
• Status: Active Grant

• Current Assignee: Leica Microsystems CMS GmbH (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Nelson, John Richard, Gao, Wei, Puleo, Christopher Michael, Miller, Todd Frederick, Pitner, Christine Lynne, Shoudy, David Andrew, Corwin, Alex David
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US14/557,169
• Publication Number:
  US 20160153868A1
• Time in Patent Office: 869 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/06 Auxiliary integrated devices, integrated components
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 1/02 Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 2001/028 Sampling from a surface, swabbing, vaporising
• CPC Class Codes: G01N 2001/4038 electric methods, e.g. electromigration, electrophoresis, ionisation

Abstract

A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

Claims

1. A system for extracting material from a region of interest, comprising:

a fluid delivery base;

a first channel within the fluid delivery base and open to a first channel end at a sample-facing surface of the fluid delivery base, wherein the first channel comprises a first channel opening configured to couple to a fluid inlet to fluidically couple the fluid inlet to the first channel end;

a second channel within the fluid delivery base and terminating at a second channel end at the sample-facing surface of the fluid delivery base, wherein the second channel comprises a second channel opening configured to couple to a fluid outlet to fluidically couple the fluid outlet to the second channel end;

an adaptor coupled to the first channel opening and the second channel opening, wherein the adaptor comprises an interior channel that couples the first channel end to the fluid inlet;

a gasket coupled to the sample-facing surface and comprising a gasket opening aligned with an area of the sample-facing surface comprising the first channel end and the second channel end;

a support comprising a sample-supporting surface configured to hold a sample against the gasket and an opposing surface; and

an alignment member coupled to the opposing surface, wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

2. The system of claim 1, wherein one or both of the alignment member or the fluid delivery base comprises a magnet.

3. The system of claim 2, wherein one or both of the fluid delivery base or the alignment member comprises a ferrous material.

4. The system of claim 1, wherein the fluid delivery base or the alignment member comprises a permanent magnet having a magnetic field that extends beyond the opposing surface of the support when the gasket is in contact with a sample disposed on the sample-supporting surface.

5. The system of claim 1, wherein one or both of the alignment member and the fluid delivery base are biased towards one another with a spring force.

6. The system of claim 1, wherein the alignment member is affixed to the support.

7. The system of claim 1, wherein the alignment member is configured to move relative to the opposing surface of the support.

8. The system of claim 1, wherein the support comprises a heating element.

9. The system of claim 1, wherein one or both of the fluid delivery base or the alignment member comprises an electromagnet coupled to a source of electric current.

10. The system of claim 1, wherein the first channel and the second channel are substantially parallel to each other.

11. The system of claim 1, wherein the first channel opening and the second channel opening are disposed on a top surface of the fluid delivery base opposing the sample-facing surface.

12. The system of claim 1, wherein the fluid delivery base comprises a light source configured to emit light from the sample facing surface within the gasket opening.

13. The system of claim 12, wherein the light source is a fiber optic light channel disposed within a third channel in the fluid delivery base.

14. The system of claim 13, wherein the third channel is between the first channel and the second channel.

15. The system of claim 1, wherein the fluid delivery base and the alignment member, when in operation, align along an axis.

16. The system of claim 15, wherein the axis intersects the gasket opening.

17. The system of claim 1, comprising the fluid inlet and the fluid outlet.

18. The system of claim 1, wherein the gasket opening defines an area less than 1 mm².

19. The system of claim 1, wherein the gasket opening defines an area greater than 1 mm².

20. The system of claim 1, wherein the gasket is positioned between the fluid delivery base and the sample-contacting surface.

21. The system of claim 1, wherein, in operation, the gasket contacts the sample.

22. The system of claim 1, wherein the opposing surface does not contact the sample.

23. The system of claim 1, wherein the sample-contacting surface forms a planar support for the sample.