Imaging systems with movable scan mirrors

• US 9,628,676 B2
• Filed: 05/31/2013
• Issued: 04/18/2017
• Est. Priority Date: 06/07/2012
• Status: Active Grant

• Current Assignee: Complete Genomics Incorporated (BGI Genomics Co., Ltd.)
• Original Assignee: Complete Genomics Incorporated (BGI Genomics Co., Ltd.)
• Inventors: Staker, Bryan P., Uhrich, Craig E.
• Primary Examiner(s): Wang, Xi
• Application Number:
  US13/907,801
• Publication Number:
  US 20140152888A1
• Time in Patent Office: 1,418 Days
• Field of Search: 348 79, 348 87, 348 80, 348 46, 396432, 359380
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/367 providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
• CPC Class Codes: G02B 27/646 compensating for small deviations, e.g. due to vibration or shake movement of one or more optical elements for control of motion blur in cameras, projectors or printers G03B2205/0007; image stabilisation in cameras peculiar to the presence or use of an electronic image sensor H04N23/68

Abstract

An imaging system is provided wherein a positioning stage is translated with respect to an objective lens component and a scan mirror is repositioned while a two-dimensional image is made of a biochemical site on a substrate. In an example embodiment, an imaging system comprises a camera, an objective lens component, a positioning stage, and a scan mirror controllable by a servo system that synchronizes movement of the positioning stage and the tilting of the scan mirror so that the substrate image is maintained stable during imaging of the continuously moving positioning stage.

Claims

1. An imaging system comprising:

a camera with a two dimensional full-frame electronic sensor;

an objective lens component configured to project an image of at least a two-dimensional portion of a substrate onto the two dimensional full-frame electronic sensor of the camera to produce a two-dimensional image;

a positioning stage configured to move in continuous motion the substrate in a first known lateral direction with respect to the objective lens component while the camera with the two dimensional full-frame electronic sensor produces the two-dimensional image;

a scan mirror configured to tilt relative to a viewing axis between the two dimensional full-frame electronic sensor and the substrate in continuous motion in coordination with the moving positioning stage, while the positioning stage moves the substrate in the first known lateral direction, in order to reflect light from the objective lens component to the camera while maintaining the substrate image stable at the electronic sensor;

a first tilt plate positioned in an optical path between the scan mirror and the camera, wherein the first tilt plate is configured to cause first offset corrections to the substrate image that is acquired by the objective lens component; and

a second tilt plate positioned in the optical path between the scan mirror and the camera, wherein the second tilt plate is configured to cause second offset corrections, in the substrate image in a second direction that is perpendicular to the first offset corrections.

2. The system of claim 1 further comprising a tube lens component positioned in an optical path between the scan mirror and the camera, wherein the scan mirror is configured to direct light from the objective lens component through the tube lens component to the two dimensional full-frame electronic sensor of the camera.

3. The system of claim 1 further comprising a light source and a dichroic mirror, wherein the dichroic mirror is configured at least to:

(a) reflect light from the light source to illuminate the substrate; and

(b) pass through light that is acquired by the objective lens component.

4. The system of claim 1 wherein movement of the positioning stage and the scan mirror are made by a servo mechanism.

5. The system of claim 1, wherein the camera is a CMOS camera.

6. An imaging system comprising:

an objective lens component that projects an array chip image of an array chip containing biochemical materials onto a full frame two-dimensional electronic sensor of a camera;

a scan mirror, capable of angular motion, that reflects light from the objective lens component to the two dimensional full-frame electronic sensor;

a positioning stage that moves the array chip in a known lateral direction with respect to the objective lens component and in coordination with the scan mirror for maintaining a stable image of the array chip at the two dimensional full-frame electronic sensor during continuous movement of the positioning stage while the camera images the array chip containing biochemical materials;

a first tilt plate between the scan mirror and the camera, the first tilt plate providing first image offset corrections; and

a second tilt plate between the scan mirror and the camera, the second tilt plate providing second image offset corrections in a direction perpendicular to the first image offset corrections.

7. The system of claim 6, wherein the array chip has DNA macromolecules disposed thereon and the camera is CMOS camera.

8. The system of claim 6 further comprising a tube lens component, wherein the scan mirror is configured to direct light from the objective lens component through the tube lens component to the two dimensional full-frame electronic sensor.

9. The system of claim 6 further comprising an angle sensor that measures an angle of the scan mirror.

10. The system of claim 9, wherein the angle sensor comprises an optical lever.

11. The system of claim 9, wherein the angle sensor comprises a laser and a linear array detector that detects light from the laser that is reflected by the scan mirror.

12. The system of claim 8 further comprising an illumination light source and a dichroic mirror disposed to illuminate the array chip while passing the array chip image to the two dimensional full-frame electronic sensor.