Single crystal quartz chips for protein crystallization and X-ray diffraction data collection and related methods

• US 9,632,042 B2
• Filed: 09/30/2014
• Issued: 04/25/2017
• Est. Priority Date: 10/25/2013
• Status: Active Grant

• Current Assignee: Renz Research, Inc.
• Original Assignee: Renz Research, Inc.
• Inventors: Ren, Zhong
• Primary Examiner(s): SONG, HOON K
• Application Number:
  US14/502,198
• Publication Number:
  US 20150117611A1
• Time in Patent Office: 938 Days
• CPC Class Codes: G01N 23/20025 Sample holders or supports therefor
• CPC Class Codes: G01N 23/207 Diffractometry using detectors, e.g. using a probe in a central position and one or more displaceable detectors in circumferential positions

Abstract

A crystallization device for in situ x-ray diffraction of biological samples includes a flat plate made of single crystal quartz, silicon or sapphire and having an array of wells or lanes defined on the plate. The crystallization device may be in the form of a cover slide used with a crystallization plate in a vapor diffusion setup. Also disclosed is a method for preparing biological samples for crystallization and x-ray diffraction, including: obtaining a crystallization device made of single crystal quartz, silicon or sapphire; loading a solution containing biological molecules onto the crystal support device; maintaining the solution in a crystal growth environment to grow crystals of the biological molecules; removing the crystal support device carrying the crystals from the crystal growth environment; exposing the crystals carried on the crystal support device to an X-ray beam; and recording X-ray diffracted by the biological crystals.

Claims

1. A crystallization device for in situ x-ray diffraction of biological samples, comprising:

a first flat plate made of single crystal quartz, single crystal silicon or single crystal sapphire and having a plurality of wells defined on the plate;

a second flat plate made of single crystal quartz, single crystal silicon or single crystal sapphire disposed to cover the first flat plate with an air gap between the first and second plates; and

a sealing material disposed between the first plate and the second plate to seal them together to define a sealed space formed by the air gap for holding the samples.

2. The crystallization device of claim 1, wherein the first plate is made of single crystal quartz, and wherein two parallel surfaces of the first plate and a bottom surface of the wells are perpendicular to a Z-axis of the quartz crystal.

3. The crystallization device of claim 2, wherein the first plate has a thickness of between 100 and 300 μ

m, and the wells have a bottom thickness of less than 50 μ

m.

4. The crystallization device of claim 2, wherein each well is a hexagon with one pair of sides parallel to an X- or Y-axis of the quartz crystal, and a diagonal of the hexagon is between 0.5 and 5 mm.

5. The crystallization device of claim 2, wherein the wells are multiple parallel lanes.

6. The crystallization device of claim 1, wherein the first plate and the second plate each has a size smaller than a 22 mm square and larger than a 1.7 mm diameter circle and a heptagon shape.

7. The crystallization device of claim 1, wherein the first plate is made of single crystal quartz, wherein two parallel surfaces of the first plate and a bottom surface of the wells are perpendicular to a Z-axis of the quartz crystal, wherein the first plate has a substantially circular shape having a diameter of about 1 inch with one straight edge parallel to an X-axis of the quartz crystal, and wherein the wells have a Y-shaped three fold symmetric pattern with one branch of the Y-shape perpendicular to the straight edge.

8. The crystallization device of claim 1, wherein the first plate further includes engraved markings indicating a coordinate system for identifying each well.

9. The crystallization device of claim 1, wherein the second plate has two parallel surfaces and is free of wells.

10. The crystallization device of claim 1, wherein the first plate and the second plate are made of the same material and have substantially identical crystal orientations.

11. The crystallization device of claim 2, wherein the second plate is made of single crystal quartz, and wherein two parallel surfaces of the second plate are perpendicular to a Z-axis of the quartz crystal.

12. A crystallization device for in situ x-ray diffraction of biological samples, comprising:

a plate made of single crystal quartz and having a plurality of wells defined on the plate, wherein two parallel surfaces of the plate and a bottom surface of the wells are perpendicular to a Z-axis of the quartz crystal, wherein the plate has a substantially circular shape having a diameter of about 1 inch with one straight edge parallel to an X-axis of the quartz crystal, and wherein the wells have a Y-shaped three fold symmetric pattern with one branch of the Y-shape perpendicular to the straight edge.