Strain of Lactobacillus rhamnosus and its metabolites for use in inhibiting xanthine oxidase and treating gout

• US 9,636,368 B2
• Filed: 08/11/2015
• Issued: 05/02/2017
• Est. Priority Date: 08/22/2014
• Status: Active Grant

• Current Assignee: Food Industry Research & Development Institute
• Original Assignee: Food Industry Research & Development Institute
• Inventors: Chen, Yen-Lin, Hsu, Hsun-Yin, Chen, Mei-Huei, Chen, Siao-Jhen, Wann, Shy-Yunn, Yu, Li-Wen
• Primary Examiner(s): Claytor, Renee
• Assistant Examiner(s): FERNANDEZ, SUSAN EMILY
• Application Number:
  US14/823,585
• Publication Number:
  US 20160051602A1
• Time in Patent Office: 630 Days
• Field of Search: None
• CPC Class Codes: A23L 2/382 fermented fermented nut meats or seeds A23L25/40; fermented milk preparations A23C9/12; addition of bacteria for nutritional purposes A23L33/135
• CPC Class Codes: A23L 2/52 Adding ingredients adding preservatives A23L2/44
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2400/175 Rhamnosus
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/0095 Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches A61K9/0007 takes precedence; eatable gels or foams A61K9/0056; oral mucosa adhesive forms A61K9/006
• CPC Class Codes: A61K 9/19 lyophilised , i.e. freeze-dried, solutions or dispersions lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00
• CPC Class Codes: A61P 19/06 Antigout agents, e.g. antihyperuricemic or uricosuric agents
• CPC Class Codes: C12N 1/02 Separating microorganisms from their culture media
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 2500/72 from bacteria
• CPC Class Codes: C12N 9/0093 acting on CH or CH2 groups (1.17)
• CPC Class Codes: C12P 1/04 by using bacteria
• CPC Class Codes: C12R 2001/225 Lactobacillus
• CPC Class Codes: C12Y 117/03002 Xanthine oxidase (1.17.3.2)

Abstract

A method for inhibiting xanthine oxidase and for reducing uric acid levels using a composition obtained by culturing Lactobacillus rhamnosus in a medium. Also disclosed is a composition including a metabolite of Lactobacillus rhamnosus for reducing uric acid levels in a subject and a method for producing the composition.

Claims

1. A method for reducing uric acid levels in a subject, the method comprising culturing Lactobacillus rhamnosus in a medium to form a composition and administering the composition to a subject in need thereof in an amount effective for reducing uric acid levels, wherein the Lactobacillus rhamnosus is removed from the composition prior to the administering step.

2. The method of claim 1, wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.

3. The method of claim 1, wherein the subject suffers from gout or hyperuricemia.

4. The method of claim 1, wherein the composition is administered orally, intramuscularly, intradermally, intravenously, subcutaneously, intraperitoneally, intranasally, or mucosally.

5. The method of claim 1, wherein the medium is de Man-Rogosa-Sharpe (MRS) broth, milk, or a juice.

6. The method of claim 1, further comprising lyophilizing the composition to form a powder.

7. A method for producing a composition for reducing uric acid levels in a subject, the method comprising inoculating a medium with Lactobacillus rhamnosus, culturing the Lactobacillus rhamnosus in the medium, removing the Lactobacillus rhamnosus from the medium to form a composition free of Lactobacillus rhamnosus, and lyophilizing the composition, wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.

8. The method of claim 7, wherein the culturing is carried out for 2 days at 37°

C.

9. The method of claim 8, wherein a culture density prior to the removing step is 1×

10⁹ cells/ml.

10. A method for producing a composition for reducing uric acid levels in a subject, the method comprising inoculating a medium with Lactobacillus rhamnosus, culturing the Lactobacillus rhamnosus in the medium, removing the Lactobacillus rhamnosus from the medium to form a composition free of Lactobacillus rhamnosus, and sterilizing the composition by pasteurization, irradiation, autoclave, or filtration, wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.