Rotating magnetic actuator

• US 9,636,689 B2
• Filed: 12/19/2013
• Issued: 05/02/2017
• Est. Priority Date: 12/21/2012
• Status: Active Grant

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Inventors: Smith, Eric, Isom, Jon, Schilffarth, Adam
• Primary Examiner(s): Mellon, David C
• Application Number:
  US14/134,042
• Publication Number:
  US 20140175019A1
• Time in Patent Office: 1,230 Days
• CPC Class Codes: B03C 1/03 rotating, e.g. of the carousel type
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/22 characterised by the magnetical field, special shape or generation

Abstract

Magnetic actuators comprising at least one linear subarray are presented. Systems comprising such magnetic actuators and methods for using such magnetic actuators to isolate magnetic particles in a fluid are also presented. Magnetic actuators comprising at least four uniform magnets are also presented, as are systems comprising such magnetic actuators and methods for using such magnetic actuators to isolate magnetic particles in a fluid.

Claims

1. A magnetic actuator configured to isolate particles in a fluid assay, the magnetic actuator comprising:

a first magnet magnetized in a first direction, a second magnet magnetized in a second direction, a third magnet magnetized in a third direction, and a fourth magnet magnetized in a fourth direction, wherein the directions are not identical and each direction is either parallel or orthogonal to the other directions such that a Halbach effect is induced on one side of the magnets;

a motor configured to rotate at least two of the first, second, third and fourth magnets;

a lateral support member configured to support the motor, the first magnet, the second magnet;

the third magnet, and the fourth magnet; and

a first linear magnet array comprising the first magnet, the second magnet, the third magnet, and the fourth magnet, where each of the first, second, third and fourth magnets is adjacent to at least one other of the first, second, third and fourth magnets;

wherein the motor is configured to rotate at least two of the first, second, third and fourth magnets in the linear magnet array approximately 180 degrees about an axis through the first magnet, the second magnet, the third magnet, and the fourth magnet.

2. The magnetic actuator of claim 1, wherein the motor is configured to rotate the first, second, third and fourth magnets in the first linear magnet array and wherein the first, second, third and fourth magnets in the first linear magnet array are configured to rotate together.

3. The magnetic actuator of claim 2, further comprising a second linear magnet array, a third linear magnet array, and a fourth linear magnet array.

4. The magnetic actuator of claim 1, wherein:

the first magnet further comprises a first axis of rotation;

the second magnet further comprises a second axis of rotation;

the third magnet further comprises a third axis of rotation;

the fourth magnet further comprises a fourth axis of rotation; and

wherein each axis of rotation is substantially parallel to the other axes of rotation and the axes of rotation are not identical.

5. The magnetic actuator of claim 4, wherein each of the first, second, third and fourth magnets is configured to rotate approximately 90 degrees and adjacent magnets of the first, second, third and fourth magnets are configured to rotate in opposite directions.

6. The magnetic actuator of claim 4, wherein two magnets of the first, second, third and fourth magnets are configured to rotate about 180 degrees, two magnets of the first, second, third and fourth magnets are configured to remain stationary, the two magnets configured to rotate about 180 degrees are not adjacent to each other, and the two magnets configured to remain stationary are not adjacent to each other.

7. A system configured to isolate particles in a fluid assay comprising:

a chassis;

a magnetic actuator according to claim 1 coupled to the chassis;

a tub coupled to the chassis; and

a well plate coupled to the tub and comprising a plurality of wells arranged in columns and rows; and

where the tub is configured to support the well plate such that at least one of the first magnet, the second magnet, the third magnet or the fourth magnet is adjacent to at least one column of wells.

8. The system of claim 7;

where the well plate further comprises eight columns and the first linear magnet array is adjacent to two columns of wells.

9. A method for collecting a sample of magnetic particles from a liquid, comprising:

obtaining a system comprising;

a chassis;

a magnetic actuator according to claim 1 coupled to the chassis;

a tub coupled to the chassis; and

a well plate coupled to the tub and comprising a plurality of wells arranged in columns and rows; and

where the tub is configured to support the well plate such that at least one of the first magnet, the second magnet, the third magnet or the fourth magnet is adjacent to at least one column of wells;

obtaining a first suspension comprising a plurality of magnetic particles suspended in a first liquid;

introducing a volume of the first suspension into at least one well of the plurality of wells;

adjusting the magnets to a first position such that a magnetic force is exerted on the at least one column of wells;

forming a pellet of magnetic particles in the at least one well of the plurality of wells; and

aspirating a portion of the first liquid from the at least one well of the plurality of wells.

10. The method of claim 9, further comprising rotating the first linear magnet array to a second position such that substantially no magnetic force is exerted on any of the columns of the plurality of wells.

11. The method of claim 10, further comprising obtaining a second liquid and introducing the second liquid into the at least one well comprising magnetic particles.

12. The method of claim 11, further comprising agitating the magnetic particles in the at least one well to form a second suspension comprising the magnetic particles suspended in the second liquid.

13. A magnetic actuator configured to isolate particles in a fluid assay, the magnetic actuator comprising:

a first magnet array comprising;

an origin; and

a linear subarray, the linear subarray comprising;

a first magnet element magnetized in a first direction relative to the origin;

a second magnet element magnetized in a second direction relative to the origin;

a third magnet element magnetized in a third direction relative to the origin; and

a fourth magnet element magnetized in a fourth direction relative to the origin;

where the first direction, the second direction, the third direction, and the fourth direction are different from one another and either substantially parallel or substantially orthogonal to one another such that a Halbach effect is induced in the linear subarray;

where the first magnet array has a length, an axis of rotation along the length of the first magnet array, a max side, and a min side;

a motor coupled to the first magnet array and configured to rotate at least one magnet element of the first, second, third and fourth magnet elements about the axis of rotation along the length of the first magnet array; and

a lateral support member configured to support the first magnet array and the motor.

14. The magnetic actuator of claim 13, further comprising a second magnet array comprising:

an origin; and

a subarray, the linear subarray comprising;

a first magnet element magnetized in a first direction relative to the origin;

a second magnet element magnetized in a second direction relative to the origin;

a third magnet element magnetized in a third direction relative to the origin; and

a fourth magnet element magnetized in a fourth direction relative to the origin;

where the first direction, the second direction, the third direction, and the fourth direction are different from one another and either substantially parallel or substantially orthogonal to one another such that a Halbach effect is induced in the linear subarray;

where the second magnet array has a length, an axis of rotation along the length of the second magnet array, a max side; and

a min side; and

where the lateral support member is further configured to support the second magnet array.

15. The magnetic actuator of claim 14, where each of the first, second, third, and fourth magnet elements in each of the first and second magnet arrays are configured to rotate together.

16. The magnetic actuator of claim 15, where the second magnet array is coupled to the first magnet array such that rotation of the first magnet array about the first axis of rotation rotates the second magnet about the second axis of rotation.

17. The magnetic actuator of claim 14, where at least some of the first, second, third, and fourth magnet elements in one of the first magnet array or the second magnet array are configured to rotate independently from others of the first;

second, third, and fourth magnet elements in the one of the first ma the second magnet array.

18. A magnetic actuator configured to isolate particles in a fluid assay, the magnetic actuator comprising:

a first pair of magnet arrays rotatable together, each magnet array of the first pair of magnet arrays comprising a plurality of magnet elements arranged to induce a Halbach effect, where each magnet array of the first pair of magnet arrays has a length, an axis of rotation, a max side, and a min side;

a first motor coupled to the first pair of magnet arrays through a gearset and configured to rotate the first pair of magnet arrays about the axis of rotation of each magnet array of the first pair of magnet arrays;

a second pair of magnet arrays rotatable together, each magnet array of the second pair of magnet arrays comprising a plurality of magnet elements arranged to induce a Halbach effect, where each magnet array of the second pair of magnet arrays has a length, an axis of rotation along the length of each magnet array of the second pair of magnet arrays, a max side, and a min side;

a second motor coupled to the second pair of magnet arrays through a gearset and configured to rotate the second pair of magnet arrays about the axis of rotation along the length of each magnet array of the second pair of magnet arrays; and

a lateral support member configured to support the magnet arrays and the motors;

where the magnetic actuator is configured to be coupled to an assay preparation module.

19. A magnetic actuator configured to isolate particles in a fluid assay, the magnetic actuator comprising:

a first uniform magnet having a length, a width, and a first axis of rotation substantially parallel to the length of the first uniform magnet, where the first uniform magnet is magnetized substantially uniformly through the width of the first uniform magnet in a first direction;

a second uniform magnet having a length, a width, and a second axis of rotation substantially parallel to the length of the second uniform magnet, where the second uniform magnet is magnetized substantially uniformly through the width of the second uniform magnet in a second direction;

a third uniform magnet having a length, a width, and a third first axis of rotation substantially parallel to the length of the third uniform magnet, where the first uniform magnet is magnetized substantially uniformly through the width of the third uniform magnet in a third direction; and

a fourth uniform magnet having a length, a width, and a fourth axis of rotation substantially parallel to the length of the fourth uniform magnet, where the fourth uniform magnet is magnetized substantially uniformly through the width of the fourth uniform magnet in a fourth direction;

wherein the first axis of rotation, the second axis of rotation, the third axis of rotation, and the fourth axis of rotation are not identical to each other and are substantially parallel to each other;

wherein the first direction, second direction, third direction, and fourth direction are not identical to each other and each direction is parallel or orthogonal to each other direction; and

wherein the uniform magnets are configured to induce a Halbach effect on the same side of all the uniform magnets;

a motor configured to rotate at least two of the uniform magnets; and

a lateral support member configured to support the uniform magnets, wherein each magnet is configured to rotate 90 degrees and adjacent magnets are configured to rotate in opposite directions.

20. The magnetic actuator of claim 19, wherein two magnets are configured to rotate about 180 degrees, two magnets are configured to remain stationary;

rotating magnets are not adjacent to each other, and stationary magnets are not adjacent to each other.