Matrices and media for storage and stabilization of biomolecules

• US 9,637,513 B2
• Filed: 01/14/2015
• Issued: 05/02/2017
• Est. Priority Date: 09/12/2008
• Status: Active Grant

• Current Assignee: GenTegra, LLC (Genisyss, LLC)
• Original Assignee: GenTegra, LLC (Genisyss, LLC)
• Inventors: Hogan, Michael, Saghbini, Michael, Wong, David
• Primary Examiner(s): THOMAS, DAVID C
• Application Number:
  US14/596,925
• Publication Number:
  US 20150158902A1
• Time in Patent Office: 839 Days
• Field of Search: None
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C09K 15/30 containing heterocyclic ring with at least one nitrogen atom as ring member
• CPC Class Codes: C12N 15/10 Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12N 5/0632 Cells of the oral mucosa
• CPC Class Codes: C12N 5/0634 Cells from the blood or the immune system
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: Y10T 428/139 Open-ended, self-supporting conduit, cylinder, or tube-type article
• CPC Class Codes: Y10T 428/1397 Single layer [continuous layer]

Abstract

The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.

Claims

1. A dry-state matrix comprising:

boric acid or a corresponding salt of boric acid; and

a plasticizer and a hydroxyl radical scavenger, wherein histidine is the plasticizer and the hydroxyl radical scavenger;

wherein the matrix is in a solid or dry form which can be converted to a liquid form by suspending, re-hydrating, or solubilizing the matrix in water.

2. The matrix of claim 1, further comprising a metal chelator.

3. The matrix of claim 1, wherein said matrix further comprises a singlet oxygen quencher selected from the group consisting of an alkyl imidazole, an indole, a sulfur-containing amino acid, a phenolic compound, an aromatic acid, azide, tocopherol, vitamin E derivatives, carotene, vitamin A derivatives, and any combination thereof.

4. The matrix of claim 1, wherein said matrix further comprises a metal chelator selected from the group consisting of EDTA, EGTA, o-phenanthroline, citrate, and any combination thereof.

5. The matrix of claim 1, wherein said matrix further comprises a hydroperoxide removing agent selected from the group consisting of catalase, pyruvate, glutathione, glutathione peroxidases, and any combination thereof.

6. The matrix of claim 1, Wherein said matrix is inert with respect to methods of nucleic acid amplification, nucleic acid digestion, and/or protein digestion.

7. The matrix of claim 1, further comprising a sample comprising biomolecules.

8. A method of storing biomolecules in a dry state, said method comprising:

mixing a sample comprising biomolecules with a dry-state matrix of claim 1 to form a mixture, and

drying said mixture to store said biomolecules in a dry state.

9. The method of claim 8, wherein said sample is a liquid sample, and wherein said liquid sample is mixed with said dry-state matrix.

10. The method of claim 8, wherein said biomolecules are stored at about 25°

C. to about 72°

C.

11. The dry-state matrix of claim 1, further comprising leucine.

12. The dry-state matrix of claim 11, further comprising a metal chelator.

13. The dry-state matrix of claim 12, wherein the metal chelator comprises EDTA.

14. The dry-state matrix of claim 13, wherein the metal chelator further comprises citrate.

15. The dry-state matrix of claim 1, wherein the dry-state matrix is paper free.

16. The dry-state matrix of claim 1, wherein the dry-state matrix is in a tube.

17. The dry-state matrix of claim 1, wherein the dry-state matrix is substantially free of cellulose.

18. The method of claim 8, wherein said dry-state matrix further comprises a metal chelator selected from the group consisting of EDTA, EGTA, o-phenanthroline, citrate, and any combination thereof.

19. The method of claim 8, wherein said dry-state matrix further comprises a hydroperoxide removing agent selected from the group consisting of catalase, pyruvate, glutathione, glutathione peroxidases, and any combination thereof.

20. The dry-state matrix of claim 1, wherein the matrix is substantially free of a biomolecule.

21. The dry-state matrix of claim 1, wherein the dry-state matrix is carried by a solid support.

22. The dry-state matrix of claim 21, wherein the solid support is selected from the group consisting of a cotton swab, a filter paper, or a sponge.