Charged triplet-state quenchers for mitigation of photo-induced damage
First Claim
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1. A reaction mixture comprising a first reactant, a second reactant comprising a fluorescent or fluorogenic label, and an additive, the additive comprising:
- a first photoprotective moiety linked to a polyanion moiety through a first linker, the first linker comprising at least one methylene group; and
a second photoprotective moiety linked to the polyanion moiety;
wherein the additive reduces an amount of photo-induced damage to the first reactant caused by interaction of the first and second reactants under excitation illumination.
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Abstract
Mitigation of photo-induced damage in excitation illuminated reactions and analyses utilizing such reactions results in an enhanced performance for the reactions and the analyses. There is provided a novel class of triplet-state quenchers for mitigating photo-induced damage which are both simple in structure and effective at preventing and/or reducing photo-induced damage to reaction components of excitation illuminated reaction mixtures. Also provided are methods of using the compounds of the invention, devices and kits incorporating the compounds of the invention.
19 Citations
25 Claims
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1. A reaction mixture comprising a first reactant, a second reactant comprising a fluorescent or fluorogenic label, and an additive, the additive comprising:
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a first photoprotective moiety linked to a polyanion moiety through a first linker, the first linker comprising at least one methylene group; and a second photoprotective moiety linked to the polyanion moiety; wherein the additive reduces an amount of photo-induced damage to the first reactant caused by interaction of the first and second reactants under excitation illumination. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for protecting an enzyme from photo-induced damage in an illuminated reaction, the method comprising:
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providing a reaction mixture comprising the enzyme, and a fluorescent or fluorogenic substrate for the enzyme, wherein interaction of the enzyme and the fluorescent or fluorogenic substrate under excitation illumination results in altered activity of the enzyme; adding an additive to the reaction mixture, the additive comprising; a first photoprotective moiety linked to a polyanion moiety through a first linker, the first linker comprising at least one methylene group; and a second photoprotective moiety linked to the polyanion moiety; and illuminating the reaction mixture with an excitation illumination, wherein the additive reduces an amount of photo-induced damage to the enzyme resulting from interaction of the enzyme with the fluorescent or fluorogenic substrate under the excitation illumination to an amount that is less than that which would occur in the absence of the additive. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 25)
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- 21. A compound having the formula:
Specification