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Tagged fragment library configured for genome or cDNA sequence analysis

  • US 9,637,785 B2
  • Filed: 08/20/2013
  • Issued: 05/02/2017
  • Est. Priority Date: 06/15/2005
  • Status: Active Grant
First Claim
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1. A DNA library configured for nucleotide sequencing of a genome, the library comprising a plurality of constructs wherein:

  • each construct contains a fragment of the genome tagged with an oligonucleotide;

    at least 96 of the constructs contain different genome fragments joined to oligonucleotide tags having different sequences;

    some of the constructs contain different genome fragments joined to oligonucleotide tags having the same sequence; and

    constructs that contain oligonucleotide tags having the same sequence more often contain fragment sequences that occur within 100 kb of each other in the genome, compared with constructs in the library that contain oligonucleotide tags having different sequences.

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