Device for transiently contacting at least one unit for capturing biological targets with a fluid containing them, method for recovering the captured targets and system for contacting and recovery
First Claim
1. A device for transient contacting notably in vivo of an one unit for capturing biological targets with a body fluid containing said targets, said device comprising:
- a sampling tip having a contacting end configured to be introduced into a medium containing said fluid, andsaid capturing unit, which is bound to said contacting end and which comprises a capture substrate defining a target capturing surface covered with a biocompatible and porous crosslinked polymer layer configured to retain said capture substrate and only to allow biological particles including said targets smaller than a limiting size to pass through, in such a way that said capture substrate is recoverable after this contacting by dissolution of said polymer layer;
wherein said capturing unit is connected to said contacting end of the tip via connecting means, which are fixed to said contacting end by welding or gluing, and said connecting means comprise a biocompatible porous bag which is fixed to said contacting end of the tip and which envelops said capturing unit, which contains particles that constitute said substrate and that are embedded in said polymer layer.
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Abstract
The invention relates to a device for transiently contacting at least one unit for capturing biological targets with a body fluid containing them, a method for recovering the captured targets for analysis, and a system for contacting and recovering a capture substrate included in said unit. The invention relates to samples obtained in particular in vivo from body fluids of the human body, e.g. circulating body fluids, said fluids possibly containing, as targets, proteins, oligonucleotides such as RNA or DNA, antibodies, enzymes or cells. A contacting device (1) according to the invention comprises: —a sampling tip (2) having a contacting end intended to be introduced into a medium containing said fluid, and—the or each capturing unit which is bound to said end and which comprises a capture substrate (3) defining a target capturing surface covered with at least one biocompatible and porous crosslinked polymer layer (4) designed to retain the or each capture substrate and only to allow biological particles including targets smaller than a limiting size to pass through, in such a way that the or each capture substrate is recoverable after said contacting by dissolution of the or each layer.
19 Citations
14 Claims
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1. A device for transient contacting notably in vivo of an one unit for capturing biological targets with a body fluid containing said targets, said device comprising:
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a sampling tip having a contacting end configured to be introduced into a medium containing said fluid, and said capturing unit, which is bound to said contacting end and which comprises a capture substrate defining a target capturing surface covered with a biocompatible and porous crosslinked polymer layer configured to retain said capture substrate and only to allow biological particles including said targets smaller than a limiting size to pass through, in such a way that said capture substrate is recoverable after this contacting by dissolution of said polymer layer; wherein said capturing unit is connected to said contacting end of the tip via connecting means, which are fixed to said contacting end by welding or gluing, and said connecting means comprise a biocompatible porous bag which is fixed to said contacting end of the tip and which envelops said capturing unit, which contains particles that constitute said substrate and that are embedded in said polymer layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification