Partitioning and processing of analytes and other species
DC CAFCFirst Claim
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1. A composition comprising a plurality of capsules, said capsules situated within droplets in an emulsion, wherein said capsules are configured to release their contents into said droplets upon the application of a stimulus to provide said contents in said droplets in said emulsion, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof.
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
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Citations
40 Claims
- 1. A composition comprising a plurality of capsules, said capsules situated within droplets in an emulsion, wherein said capsules are configured to release their contents into said droplets upon the application of a stimulus to provide said contents in said droplets in said emulsion, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof.
- 22. A composition comprising a plurality of outer capsules in an emulsion, said outer capsules comprising at least one inner capsule, wherein said at least one inner capsule is configured to release its contents into at least one outer capsule among said plurality of outer capsules upon the application of a stimulus to provide said contents in said outer capsule in said emulsion, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof.
- 23. A device comprising a plurality of partitions, wherein at least one partition of said plurality of partitions comprises a capsule, wherein said capsule is situated within a droplet in an emulsion, wherein said capsule is configured to release its contents into said droplet upon the application of a stimulus to provide said contents in said droplet in said emulsion, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof.
- 24. A device comprising a plurality of partitions, wherein at least one partition of said plurality of partitions comprises an outer capsule in an emulsion, said outer capsule comprising at least one inner capsule, wherein said at least one inner capsule is configured to release its contents into said outer capsule upon the application of a stimulus to provide said contents in said outer capsule in said emulsion, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof.
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25. A method comprising:
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a. providing a plurality of capsules, said capsules situated within droplets in an emulsion, wherein said capsules are configured to release their contents into said droplets upon the application of a stimulus, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof; and b. providing a stimulus to cause said capsules to release their contents into said droplets in said emulsion. - View Dependent Claims (31)
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26. A method comprising:
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a. providing a plurality of inner capsules, said inner capsules situated within outer capsules in an emulsion, wherein said inner capsules are configured to release their contents into said outer capsules upon the application of a stimulus, wherein said stimulus is selected from the group consisting of a change in pH, a change in ion concentration, reduction of disulfide bonds, and combinations thereof; and b. providing a stimulus to cause said inner capsules to release their contents into said outer capsules in said emulsion. - View Dependent Claims (32)
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Specification