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Methods and compositions for incorporating nucleotides

  • US 9,644,237 B2
  • Filed: 05/02/2016
  • Issued: 05/09/2017
  • Est. Priority Date: 03/19/2008
  • Status: Active Grant
First Claim
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1. A composition comprising a mixture of labeled and unlabeled reversible terminators in solution, said terminators comprising nucleotide analogues of dCTP, dGTP and dATP, wherein the concentration of the unlabeled nucleotide analogue of dCTP is present in said solution at a higher concentration than the labeled nucleotide analogue of dCTP, wherein the concentration of the unlabeled nucleotide analogue of dGTP is present in said solution at a higher concentration than the labeled nucleotide analogue of dGTP, wherein the concentration of the unlabeled nucleotide analogue of dATP is present in said solution at a higher concentration than the labeled nucleotide analogue of dATP, and wherein each labeled and unlabeled nucleotide analogue contains a removable chemical moiety capping the 3′

  • -OH group.

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