Monolithically integrated hybridisation sensor assembly and associated production method
First Claim
1. A monolithically integrated sensor assembly configured for detecting DNA single strands possibly contained in an analyte, comprising:
- a substrate;
at least one sensor electrode arranged at least one of on and in the substrate, and coated with a sensor-active layer at which are generated electrochemically active catcher DNA single strands in the presence of the DNA single strands to be detected, the electrochemically active catcher DNA single strands being detectable by producing an electrical sensor signal based on DNA single strands detected by the at least one sensor electrode;
a plurality of comparison electrodes, at which a plurality of electrical comparison signals independent of the electrical sensor signal is detected, wherein the plurality of comparison electrodes is configured for measuring the electrical comparison signal simultaneously with measurement of the electrical sensor signal detected by the at least one sensor electrode in the presence of the DNA single strands to be detected, and the electrical comparison signal is at least one selected from a background signal, a noise signal and a zero signal which do not originate from the sensor event; and
an operating circuit integrated at least one of on and in the substrate, and configured for driving the at least one sensor electrode, the operating circuit including at least one evaluation unit,wherein said evaluation unit is configured to carry out a statistical averaging of the comparison signals detected from the plurality of comparison electrodes and to jointly evaluate the sensor signal and the statistical averaging of the comparison signals so as to eliminate undesirable disturbing contributions whereby detection sensitivity is increased, signal-to-noise ratio improved, and noise margin increased.
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Accused Products
Abstract
A monolithically integrated sensor assembly is for detecting molecules that are potentially contained in an analyte. The assembly includes: a substrate; at least one sensor electrode, which is located on or in the substrate and is coated with a sensor-active layer, on which electrochemically active particles are generated in the present of the molecules to be detected, the particles being detected via an electric sensor signal on the sensor electrode(s); at least one additional electrode that is located on or in the substrate; and an operating circuit that is integrated into the substrate for controlling the sensor electrode and the additional electrode(s).
6 Citations
24 Claims
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1. A monolithically integrated sensor assembly configured for detecting DNA single strands possibly contained in an analyte, comprising:
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a substrate; at least one sensor electrode arranged at least one of on and in the substrate, and coated with a sensor-active layer at which are generated electrochemically active catcher DNA single strands in the presence of the DNA single strands to be detected, the electrochemically active catcher DNA single strands being detectable by producing an electrical sensor signal based on DNA single strands detected by the at least one sensor electrode; a plurality of comparison electrodes, at which a plurality of electrical comparison signals independent of the electrical sensor signal is detected, wherein the plurality of comparison electrodes is configured for measuring the electrical comparison signal simultaneously with measurement of the electrical sensor signal detected by the at least one sensor electrode in the presence of the DNA single strands to be detected, and the electrical comparison signal is at least one selected from a background signal, a noise signal and a zero signal which do not originate from the sensor event; and an operating circuit integrated at least one of on and in the substrate, and configured for driving the at least one sensor electrode, the operating circuit including at least one evaluation unit, wherein said evaluation unit is configured to carry out a statistical averaging of the comparison signals detected from the plurality of comparison electrodes and to jointly evaluate the sensor signal and the statistical averaging of the comparison signals so as to eliminate undesirable disturbing contributions whereby detection sensitivity is increased, signal-to-noise ratio improved, and noise margin increased. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 21, 24)
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16. A sensor array, comprising a plurality of monolithically integrated sensor assemblies, each of which is formed at least one of on and in a substrate, each of the monolithically integrated sensor assemblies comprising:
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at least one sensor electrode arranged at least one of on and in the substrate, and coated with a sensor-active layer at which are generated electrochemically active catcher DNA single strands in the presence of the DNA single strands to be detected, the electrochemically active catcher DNA single strands being detectable by producing an electrical sensor signal based on DNA single strands detected by the at least one sensor electrode; and a plurality of comparison electrodes, at which a plurality of electrical comparison signals independent of the electrical sensor signal are detected, wherein the plurality of comparison electrodes is configured for measuring the electrical comparison signal simultaneously with measurement of the electrical sensor signal detected by the at least one sensor electrode in the presence of the DNA single strands to be detected, and the electrical comparison signal is at least one selected from a background signal, a noise signal and a zero signal which do not originate from the sensor event. - View Dependent Claims (17, 18, 20)
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19. A method for producing a monolithically integrated sensor assembly configured for detecting DNA single strands possibly contained in an analyte, the method comprising:
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forming, at least one of on and in a substrate, at least one sensor electrode coated with a sensor-active layer at which are generated electrochemically active catcher DNA single strands in the presence of the DNA single strands contained in the analyte to be detected, the electrochemically active catcher DNA single strands being detectable by producing an electrical sensor signal detected by the at least one sensor electrode; forming at plurality of comparison electrode for producing a plurality of comparison signals from exposure to the analyte simultaneously with the electrical sensor signal detected by the at least one sensor electrode in the presence of the DNA single strands to be detected, the plurality of electrical comparison signals being at least one selected from a background signal, a noise signal and a zero signal which do not originate from the sensor event; and integrating an operating circuit, configured for driving the at least one sensor electrode, the operating circuit including at least one evaluation unit by which a sensor event is determined at the at least one sensor electrode based on the electrical sensor signal and the plurality of electrical comparison signal, wherein the comparison electrode lacks the sensor-active layer provided on the sensor electrode, wherein the operating circuit is operative for producing statistical averaging of the plurality of the electrical comparison signals and a difference signal from the signals of the measuring electrode and the statistical averaging as a measurement signal which is free of undesirable disturbing contributions based on charge reversals of double-layer capacitances at the electrode surfaces. - View Dependent Claims (22, 23)
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Specification