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Electrochemical methods and devices for amending urine samples for immunosensor detection

  • US 9,651,547 B2
  • Filed: 03/12/2014
  • Issued: 05/16/2017
  • Est. Priority Date: 03/14/2013
  • Status: Expired due to Fees
First Claim
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1. A device configured to perform an immunoassay for a target analyte in an amended urine sample, the device comprising:

  • a first region comprising reagents for of amending a urine sample, wherein the reagents comprise a water soluble protein for blocking non-specific binding of immunoglobulins at a sensor and a buffer; and

    a second region comprising;

    (i) a capture molecule capable of binding to the target analyte bound to a conjugate molecule labeled with an enzyme to form an immunocomplex, and (ii) the sensor comprising at least one electrode configured to detect the immunocomplex and determine a concentration of the target analyte in the amended urine sample,wherein the first region is configured to provide a dissolved concentration of the water soluble protein within the amended urine sample in a range of about 0.02 to 225 mg/mL; and

    wherein the reagents further comprise a scavenger for reducing non-specific current generation from electroactive species at the at least one electrode.

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