Method and kit for the detection of hepatitis-specific antibodies

• US 9,651,557 B2
• Filed: 03/07/2013
• Issued: 05/16/2017
• Est. Priority Date: 03/08/2012
• Status: Active Grant

• Current Assignee: Dobroslav Melamed, Dror Atzmon
• Original Assignee: Dobroslav Melamed, Dror Atzmon
• Inventors: Jehuda-Cohen, Tamar
• Primary Examiner(s): CHEN, STACY BROWN
• Application Number:
  US14/383,574
• Publication Number:
  US 20150111767A1
• Time in Patent Office: 1,531 Days
• Field of Search: None
• CPC Class Codes: G01N 33/576 for hepatitis
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates to an improved method for detecting antibodies to a hepatitis virus in a tissue sample from individuals, which can reliably detect antibodies in recently infected individuals and which provides much lower false positive results in individuals that have cleared their hepatitis infections. More particularly, the present invention relates to an improved method and kit which utilizes an activator of (i) hepatitis virus-primed lymphocytes, (ii) memory cells specific for said hepatitis virus, (iii) hepatitis virus-specific antibody production, or (iv) a combination thereof in a tissue sample to stimulate the production of antibodies from newly primed B cells, if present.

Claims

1. A kit for the detection of hepatitis virus-specific antibodies in a subject comprising:

a container for retaining a tissue sample, wherein said container comprises a medium, said medium comprising a) one or more activators of (i) lymphocytes activated by said hepatitis virus, (ii) memory cells specific for said hepatitis virus, (iii) antibodies against said hepatitis virus, or (iv) a combination thereof, and b) an anti-CD19 antibody against a B-lymphocyte membrane domain.

2. The kit according to claim 1, wherein said kit further comprises a second container lacking said medium for retaining a second tissue sample.

3. The kit according to claim 1, wherein said tissue sample is a whole blood sample.

4. The kit according to claim 1, wherein said container (a) is made of a plastic, glass, silicon, synthetic membrane, or metal, (b) is a test tube, a bottle, a well or a flask, (c) is vacuum sealed, (d) is a vacutube, or (e) comprises a polystyrene polymer, a polypropylene polymer, or a combination thereof.

5. The kit according to claim 1, wherein said container has a cap, stopper, or lid that has first and second positioning mechanism, wherein said first positioning mechanism renders the container fully closed, thereby maintaining liquid and vacuum pressure inside said container and wherein said second positioning mechanism provides ventilation to the contents of said container while preserving a sterile environment inside said container.

6. The kit according to claim 1, wherein said hepatitis virus is a hepatitis C virus (HCV).

7. The kit according to claim 1, wherein said hepatitis virus is a hepatitis B virus (HBV), a hepatitis A virus (HAV), a hepatitis D virus (HDV), or a hepatitis E virus (HEV).

8. The kit according to claim 1, wherein said medium comprises a non-carbon dioxide-dependent buffering agent.

9. The kit according to claim 8, wherein said buffering agent is HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) or a phosphate-based buffering agent.

10. The kit according to claim 1, wherein said activator is pokeweed mitogen.

11. The kit according to claim 1, wherein said activator is a viral-derived peptide, lectin, bacterial endotoxin, a virus, lipid A, a cytokine, a chain of nucleotides, or a lymphokine.

12. The kit according to claim 1, wherein said lymphocytes activated by said hepatitis virus or memory cells are B-lymphocytes.

13. The kit according to claim 1, wherein said lymphocytes activated by said hepatitis virus or memory cells are T-lymphocytes.

14. The kit according to claim 1, wherein said kit is used in an assay for the detection of hepatitis viral-specific antibodies.

15. The kit according to claim 14, wherein said assay is an enzyme linked immunosorbent assay, a western blot, or an immunofluorescence assay.

16. The kit according to claim 14, wherein said kit comprises a solid phase support.

17. The kit according to claim 16, wherein said solid phase support is a nitrocellulose strip, polymer beads, synthetic beads, or a combination thereof.

18. The kit according to claim 1, further comprising a needle for drawing blood from said subject.