Reduction of gastrointestinal tract colonisation by campylobacter

• US 9,655,912 B2
• Filed: 02/15/2013
• Issued: 05/23/2017
• Est. Priority Date: 02/16/2012
• Status: Active Grant

• Current Assignee: Akeso Biomedical, Inc.
• Original Assignee: Akeso Biomedical, Inc.
• Inventors: Mahdavi, Jafar, Ala'Aldeen, Dlawer
• Primary Examiner(s): Maier, Leigh C
• Application Number:
  US14/379,473
• Publication Number:
  US 20150025026A1
• Time in Patent Office: 1,558 Days
• CPC Class Codes: A23K 20/111 Aromatic compounds
• CPC Class Codes: A23K 20/142 Amino acids; Derivatives thereof
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A61K 31/132 having two or more amino groups, e.g. spermidine, putrescine
• CPC Class Codes: A61K 31/136 having the amino group directly attached to the aromatic ring, e.g. benzeneamine
• CPC Class Codes: A61K 31/164 of a carboxylic acid with an aminoalcohol, e.g. ceramides
• CPC Class Codes: A61K 31/191 having two or more hydroxy groups, e.g. gluconic acid
• CPC Class Codes: A61K 31/198 Alpha-amino acids, e.g. alanine or edetic acid [EDTA] betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00
• CPC Class Codes: A61K 31/295 Iron group metal compounds
• CPC Class Codes: A61K 31/335 having oxygen as the only ring hetero atom, e.g. fungichromin
• CPC Class Codes: A61K 31/555 containing heavy metals, e.g. hemin, hematin, melarsoprol
• CPC Class Codes: A61K 31/661 Phosphorus acids or esters thereof not having P—C bonds, e.g. fosfosal, dichlorvos, malathion or mevinphos
• CPC Class Codes: A61K 31/7008 Compounds having an amino group directly attached to a carbon atom of the saccharide radical, e.g. D-galactosamine, ranimustine
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Campylobacter are the commonest reported bacterial causes of gastroenteritis in the UK and industrialized worlds. This invention relates to a method of preventing or reducing the colonization of the gastrointestinal tract of an animal with Campylobacter. Accordingly, the present invention provides a method for disinfection of an animal comprising administering to said animal at least one compound that binds to MOMP or FlaA of Campylobacter in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of said animal. The present invention also provides a method of preventing or reducing transmission of Campylobacter from one animal to another.

Claims

1. A method for treating or reducing Campylobacter colonization in an animal or meat product comprising administering to the animal or the meat product at least one compound in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of the animal or in the meat product,

wherein the compound is selected from the group consisting of:

2. The method of claim 1, wherein the Campylobacter is Campylobacter jejuni or Campylobacter Coli.

3. The method of claim 1, wherein the compound is administered orally.

4. The method of claim 1, wherein the compound specifically binds to at least one of amino acid residue selected from the group consisting of Arg^3S2, Thr²⁶⁸, Lys²⁷⁸, Lys³⁸⁵, Asn²⁵⁸, Leu²⁹⁰, Tyr²⁹⁴, Phe³⁹⁵, Ile³³⁷, Arg³⁸¹, Asp²⁶¹, and Ser³⁹⁷ of MOMP (SEQ ID NO:

1).

5. The method of claim 1, wherein the compound specifically binds to at least amino acid residue Thr²⁶⁸ of MOMP (SEQ ID NO. 1).

6. The method of claim 1, wherein the compound is in the form of an animal feed, animal drinking water, feed ingredient or feed supplement comprising the compound.

7. The method of claim 1, wherein the compound is administered in an animal'"'"'s feed or drinking water.

8. The method of claim 1, wherein the compound is administered daily between 3 and 5 days before slaughter of a non-human animal.

9. The method of claim 1, wherein the compound is administered in an amount of between 0.3 and 32 mg/day/kilogram body weight of the animal.

10. The method of claim of claim 1 further comprising the steps of preparing a meat product from a non-human animal.

11. The method of claim 10, wherein the animal is poultry.

12. The method of claim 1 comprising administering the compound to a human.

13. The method of claim 1, wherein the compound reduces colonisation of the gastrointestinal tract of the animal with Campylobacter.

14. The method of claim 13, wherein the animal is poultry for human consumption.

15. The method of claim 1, wherein the animal is selected from the group consisting of cattle, sheep, pigs, goats and deer.

16. The method of claim 11, wherein the poultry is selected from the group consisting of chickens, geese, turkeys and ducks.

17. A method for treating or reducing Campylobacter colonization in an animal or meat product comprising administering to a non-human animal or a non-human meat product ferric quinate in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of the animal or in the meat product.

18. The method of claim 17, wherein the Campylobacter is Campylobacter jejuni or Campylobacter Coli.

19. The method of claim 17, wherein the compound is administered orally.

20. The method of claim 19, wherein the compound is in the form of an animal feed, animal drinking water, feed ingredient or feed supplement comprising the compound.

21. The method of claim 17, wherein the compound is administered in the animal'"'"'s feed or drinking water.

22. The method of claim 17, wherein the compound is administered daily between 3 and 5 days before slaughter of the animal.

23. The method of claim 17, wherein the compound is administered in an amount of between 0.3 and 32 mg/day/kilogram body weight of the animal.

24. The method of claim of claim 17 further comprising the step of preparing a meat product from the animal.

25. The method of claim 17, wherein the animal is selected from the group consisting of cattle, sheep, pigs, goats and deer.

26. The method of claim 17, wherein the animal is poultry selected from the group consisting of chickens, geese, turkeys and ducks.