Anti-adhesive barrier membrane using alginate and hyaluronic acid for biomedical applications

• US 9,656,001 B2
• Filed: 07/20/2015
• Issued: 05/23/2017
• Est. Priority Date: 10/08/2010
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Schmidt, Christine E., Mayes, Sarah
• Primary Examiner(s): BASQUILL, SEAN M
• Application Number:
  US14/803,258
• Publication Number:
  US 20160015866A1
• Time in Patent Office: 673 Days
• CPC Class Codes: A61K 31/192 having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid cannabinoids A61K31/658
• CPC Class Codes: A61K 31/195 having an amino group
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/43 Hormones, e.g. dexamethasone
• CPC Class Codes: A61L 2300/62 Encapsulated active agents, e.g. emulsified droplets
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 31/145 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 31/148 Materials at least partially resorbable by the body
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C08L 5/04 Alginic acid; Derivatives thereof
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof

Abstract

A non-synthetic, hydrophilic, biodegradable, biocompatible polysaccharide based non-toxic anti-adhesion hydrogel barrier is disclosed herein. The barrier of the present invention is formed by constructing a unique interpenetrating, crosslinked network with a unique porosity. Furthermore, the barrier of the present invention is comprised of tunable biopolymers for controllable mechanical robustness and degradation. The barrier of the present invention effectively reduces unwanted adhesions using non-synthetic components.

Claims

1. An apparatus comprising:

a translucent, desiccated, amorphous, non-synthetic, non-cytotoxic hydrogel film including;

a first film layer comprising first uncrosslinked hyaluronic acid and first alginate, the first alginate being negatively charged and crosslinked with at least one first cation; and

a second film layer comprising second uncrosslinked hyaluronic acid and second alginate, the second alginate being negatively charged and crosslinked with at least one second cation;

wherein (a) the first film layer is fused to the second film layer, (b) each of the at least one first and second cations includes calcium;

(c) the first film layer is bonded to a surface coating comprising ester bonds and one biopolymer selected from the group consisting of collagen, chitosan, gelatin, and combinations thereof;

(d) the composition of each of the first and second film layers is no less than 1% hyaluronic acid by dry weight and no greater than 33% hyaluronic acid by dry weight; and

(e) at least one of the first and second film layers is adhesive to cells and another of the first and second film layers is anti-adhesive and non-attractive to cells.

2. The apparatus of claim 1, wherein the first hyaluronic acid and the first alginate are formed around a pore network.

3. The apparatus of claim 2, wherein the pore network is branched.

4. The apparatus of claim 1 wherein the hydrogel film is configured to prevent tissue adhesion following surgery.

5. The apparatus of claim 1, wherein the hydrogel film encapsulates at least one of a drug, growth factor, hormone, protein, and combinations thereof.

6. The apparatus of claim 1, wherein the hydrogel film is unpatterned.

7. The apparatus of claim 2, wherein the pore network includes channels comprising at least one of a drug, growth factor, hormone, protein, and combinations thereof.

8. The apparatus of claim 1, wherein the first film layer is anti-adhesive and non-attractive to cells and the second film layer is adhesive to cells.