DNA analyzer
First Claim
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1. A microfluidic chip, comprising:
- an inlet configured to receive a template DNA extracted from a sample acceptor that is external of the microfluidic chip and is fluidically coupled to the microfluidic chip on a cartridge;
a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, the first domain including a thermal-coupler reservoir coupled with a PCR reservoir for PCR amplification, thermal cycles in the PCR reservoir being induced based on temperature measured from the thermal-coupler reservoir;
a dilution domain having a reservoir that is fluidically coupled to the first domain and is configured to dilute a PCR mixture received from the first domain with a dilutant in the reservoir; and
a second domain that is fluidically coupled to the dilution domain so as to receive the amplified DNA fragments, the second domain including a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments.
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Abstract
Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.
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Citations
19 Claims
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1. A microfluidic chip, comprising:
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an inlet configured to receive a template DNA extracted from a sample acceptor that is external of the microfluidic chip and is fluidically coupled to the microfluidic chip on a cartridge; a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, the first domain including a thermal-coupler reservoir coupled with a PCR reservoir for PCR amplification, thermal cycles in the PCR reservoir being induced based on temperature measured from the thermal-coupler reservoir; a dilution domain having a reservoir that is fluidically coupled to the first domain and is configured to dilute a PCR mixture received from the first domain with a dilutant in the reservoir; and a second domain that is fluidically coupled to the dilution domain so as to receive the amplified DNA fragments, the second domain including a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A cartridge, comprising:
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a sample acceptor external to and fluidically coupled to a microfluidic chip on the cartridge, the sample acceptor configured to extract a template DNA and the microfluidic chip having a first domain and a second domain coupled to the first domain, wherein the first domain is configured to perform polymerase chain reaction (PCR) amplification of DNA fragments based on the template DNA, and the second domain has a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments, the first domain includes a thermal-coupler reservoir coupled with a PCR reservoir for PCR amplification, thermal cycles in the PCR reservoir are induced based on temperature measured from the thermal-coupler reservoir. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification