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Method of making a paired tag library for nucleic acid sequencing

  • US 9,657,291 B2
  • Filed: 07/14/2015
  • Issued: 05/23/2017
  • Est. Priority Date: 01/09/2008
  • Status: Active Grant
First Claim
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1. A plurality of circular nucleic acid molecules, the individual circular molecules in the plurality comprising:

  • (a) a first and a second adapter sequence common to the plurality of circular nucleic acid molecules, wherein each of the first and the second adapter sequences are less than 100 bases in length; and

    (b) a target sequence region that differs between different molecules of the plurality of circular molecules, wherein each target sequence region comprises a fragment of genomic DNA comprising between 200 bp and 15 kb, wherein each of the circular nucleic molecules comprise a first and second nick in the target sequence, one on each strand of the circular nucleic acid molecule, wherein the first and second nick are each located at different positions within the target sequence region.

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