×

Proximity assay for in situ detection of targets

  • US 9,663,814 B2
  • Filed: 03/11/2014
  • Issued: 05/30/2017
  • Est. Priority Date: 03/12/2013
  • Status: Active Grant
First Claim
Patent Images

1. A method for analyzing a sample to determine whether a first target is proximal to a second target, the method comprising:

  • contacting a sample with a first conjugate comprising a biotin ligase and a first binding moiety specific for the first target;

    contacting the sample with a second conjugate comprising a biotin ligase peptide substrate and a second binding moiety specific for the second target;

    contacting the sample with biotin and ATP so that the biotin ligase labels the biotin ligase peptide substrate with biotin if the first target is proximal to the second target; and

    detecting the biotin label;

    wherein the first and second binding moieties are selected from an antibody, an antibody fragment, or an antibody derivative or an antibody mimetic.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×