Assay plates, reader systems and methods for luminescence test measurements
First Claim
1. A multi-well plate comprising a plate top having plate top openings and a plate bottom mated to said plate top to define wells of said multi-well plate, said plate bottom comprising a substrate having a top surface with electrodes patterned thereon and a bottom surface with electrical contacts patterned thereon, said electrical contacts being positioned on said bottom surface between said wells of said multi-well plate, wherein said electrodes and contacts are patterned such that each well comprises:
- (a) a working electrode on said top surface of said substrate, wherein said working electrode is electrically connected to a first of said electrical contacts; and
(b) a counter electrode on said top surface of said substrate, wherein said counter electrode is electrically connected with a second of said electrical contacts and said working and counter electrodes are electrically isolated, wherein said plate bottom comprises an insulating substrate and a conductive substrate, wherein said conductive substrate is folded around said insulating substrate forming said electrodes on a first side of said insulating substrate and said electrical contacts on a second side of said insulating substrate.
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Abstract
Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
53 Citations
43 Claims
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1. A multi-well plate comprising a plate top having plate top openings and a plate bottom mated to said plate top to define wells of said multi-well plate, said plate bottom comprising a substrate having a top surface with electrodes patterned thereon and a bottom surface with electrical contacts patterned thereon, said electrical contacts being positioned on said bottom surface between said wells of said multi-well plate, wherein said electrodes and contacts are patterned such that each well comprises:
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(a) a working electrode on said top surface of said substrate, wherein said working electrode is electrically connected to a first of said electrical contacts; and (b) a counter electrode on said top surface of said substrate, wherein said counter electrode is electrically connected with a second of said electrical contacts and said working and counter electrodes are electrically isolated, wherein said plate bottom comprises an insulating substrate and a conductive substrate, wherein said conductive substrate is folded around said insulating substrate forming said electrodes on a first side of said insulating substrate and said electrical contacts on a second side of said insulating substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification