Method for measuring temperature of biological sample, measuring device, and biosensor system

• US 9,664,639 B2
• Filed: 06/15/2016
• Issued: 05/30/2017
• Est. Priority Date: 01/30/2009
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Fujiwara, Masaki
• Primary Examiner(s): Wallenhorst, Maureen
• Application Number:
  US15/182,836
• Publication Number:
  US 20160305901A1
• Time in Patent Office: 349 Days
• Field of Search: 436 63, 436 70, 436 95, 436147, 436149, 436150, 422 681, 422 73, 422 8201, 422 8202, 422 8212, 422503, 435 14, 435 29, 20440301, 205792
• CPC Class Codes: A61B 2560/0252 using ambient temperature
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01K 7/00 Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat giving results other than momentary value of temperature G01K3/00 ; Power supply therefor, e.g. using thermoelectric elements
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/28 Electrolytic cell components
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

The concentration measurement method includes: introducing a predetermined amount of the biological sample into the capillary; measuring a temperature of the biological sample by applying a first voltage to the electrode unit when the temperature of the biological sample is measured, the first voltage allowing the temperature measurement to be less affected by increase and reduction in an amount of the analyte contained in the biological sample; measuring the concentration of the analyte contained in the biological sample by applying a second voltage to the electrode unit; measuring an environmental temperature in a surrounding of the biological sample; and correcting the concentration of the measured analyte based on the measured temperature of the biological sample and the measured environmental temperature.

Claims

1. A concentration measurement method of measuring a concentration of an analyte contained in a biological sample in a sensor chip including:

an electrode unit including a working electrode and a counter electrode, the electrode unit including a reagent containing an electrolyte, and a capillary allowing the biological sample to be introduced therein, the concentration measurement method comprising;

an introduction of introducing a predetermined amount of the biological sample into the capillary;

a temperature measurement of measuring a temperature of the biological sample by applying a first voltage to the electrode unit when the temperature of the biological sample is measured, the first voltage having a potential difference of 1.0 V or greater;

a concentration measurement of measuring the concentration of the analyte contained in the biological sample by applying a second voltage to the electrode unit, the first voltage being greater than the second voltage;

wherein the temperature measurement is executed at least twice in the temperature measurement of measuring a temperature.

2. The concentration measurement method according to claim 1,

wherein the temperature measurement of measuring temperature is executed during the concentration measurement of measuring the concentration.

3. The concentration measurement method according to claim 2, the concentration measurement method further comprising:

a correction of correcting the concentration of the analyte measured in the concentration measurement based on the temperature of the biological sample measured in the temperature measurement.

4. The concentration measurement method according to claim 2,

wherein the concentration measurement includes applying the second voltage to a measurement electrode that is separate from the electrode unit, and the temperature measurement is executed independently from the concentration measurement.

5. The concentration measurement method according to claim 4,

wherein an order and a timing of the application of the first voltage in the temperature measurement is arbitrarily determined with respect to the application of the second voltage in the concentration measurement.

6. The concentration measurement method according to claim 1,

wherein the temperature measurement of measuring temperature is executed immediately after starting the concentration measurement of measuring the concentration and immediately before completing concentration measurement of measuring the concentration.

7. The concentration measurement method according to claim 1,

wherein the temperature measurement of measuring temperature is executed before and after the concentration measurement of measuring the concentration.

8. The concentration measurement method according to claim 1,

wherein the concentration measurement includes measuring the concentration of the analyte by measuring at least one of concentrations of glucose, hematocrit and reducing substance.

9. The concentration measurement method according to claim 1,

wherein the temperature measurement is executed after the concentration measurement is completed.

10. A measuring device for measuring a concentration of an analyte contained in a biological sample, wherein the measuring device is configured to apply a voltage to a sensor chip including an electrode unit including a working electrode and a counter electrode, the electrode unit including a reagent containing an electrolyte, the measuring device comprising;

an insertion section allowing the sensor chip to be loaded therein;

a voltage application section configured to apply a first voltage to the electrode unit of the sensor chip loaded into the insertion section, the first voltage having a potential difference of 1.0 V or greater; and

a temperature measurement section configured to measure a temperature of the biological sample located in the sensor chip based on an output value of the first voltage applied by the voltage application section,

wherein the temperature measurement section is configured to execute a temperature measurement at least twice.

11. The measuring device according to claim 10,

wherein the voltage application section is configured to apply a direct-current voltage falling in a voltage range allowing the biological sample to be electrolyzed.

12. The measuring device according to claim 10, further comprising:

the voltage application section configured to apply a second voltage to the electrode unit; and

an analyte measurement section configured to measure a concentration of the analyte contained in the biological sample based on an output value of the second voltage applied by the voltage application section.

13. The measuring device according to claim 12,

wherein the analyte measurement section is configured to measure the concentration of the analyte by measuring at least one of concentrations of glucose, hematocrit and reducing substance.

14. The measuring device according to claim 12,

wherein the first voltage applied by the voltage application section in the temperature measurement is greater than the second voltage applied by the voltage application section in the concentration measurement.