Stevia Cultivar ‘814011’

• US 9,668,451 B2
• Filed: 08/31/2015
• Issued: 06/06/2017
• Est. Priority Date: 09/02/2014
• Status: Active Grant

• Current Assignee: PureCircle USA Inc. (Ingredion Incorporated)
• Original Assignee: PureCircle USA Inc. (Ingredion Incorporated)
• Inventors: Bu, Yu Cheng, Markosyan, Avetik, Li, Shan Wang
• Primary Examiner(s): Kruse, David H
• Application Number:
  US14/841,593
• Publication Number:
  US 20160057966A1
• Time in Patent Office: 645 Days
• Field of Search: None
• CPC Class Codes: A01H 5/12 Leaves
• CPC Class Codes: A01H 6/1488 Stevia
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

A stevia cultivar, designated ‘814011’, is disclosed. The invention relates to the plant parts of stevia cultivar ‘814011’, to the plants of stevia ‘814011’ and to methods for producing a stevia plant produced by crossing the cultivar ‘814011’ with itself or another stevia variety, including methods using marker assisted breeding. The invention further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘814011’ with another stevia cultivar.

Claims

1. A plant of Stevia rebaudiana cultivar ‘

814011’

, wherein a representative sample of live plant tissue of said cultivar was deposited under CGMCC No. 9701.

2. A plant, or a plant part thereof consisting of leaves, cotyledons, hypocotyl, meristematic cells, ovules, seeds, cells, roots, root tips, pistils, anthers, flowers, and stems, produced by growing the plant of claim 1.

3. A stevia plant, or part thereof, having all of the physiological and morphological characteristics of the stevia plant of claim 1.

4. A method of producing a food or feed product, said method comprising obtaining the plant or plant part of claim 2, and producing said product.

5. A tissue or cell culture of regenerable cells produced from the plant of claim 1.

6. The tissue or cell culture of claim 5, comprising tissues or cells from a plant part selected from the group consisting of leaves, cotyledons, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, and stems.

7. A stevia plant regenerated from the tissue or cell culture of claim 6, wherein said plant has all of the morphological and physiological characteristics of stevia cultivar ‘

814011’

listed in Table 1.

8. A method of vegetatively propagating a stevia plant, said method comprising the steps of:

p1 a. collecting tissue or cells capable of being propagated from the plant according to claim 1;

b. cultivating said tissue or cells of (a) to obtain proliferated shoots; and

c. rooting said proliferated shoots to obtain rooted plantlets;

or

d. cultivating said tissue or cells to obtain proliferated shoots, or to obtain plantlets.

9. A stevia plant produced by growing the plantlets or proliferated shoots of claim 8.

10. A method for producing an F₁ stevia seed, wherein the method comprises crossing the plant of claim 1 with a different stevia plant and harvesting the resultant F₁ stevia seed.

11. A stevia seed produced by the method of claim 10.

12. A stevia plant, or a part thereof, produced by growing said seed of claim 11.

13. A method of determining the genotype of the stevia plant of claim 1, wherein said method comprises obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms.

14. A method of producing an herbicide resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene wherein the transgene confers resistance to an herbicide chosen from glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, cyclohexanedione, L-phosphinothricin, triazine, benzonitrile, and bromoxynil.

15. An herbicide resistant stevia plant produced by the method of claim 14.

16. A method of producing an insect resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene that confers insect resistance.

17. An insect resistant stevia plant produced by the method of claim 16.

18. The stevia plant of claim 17, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

19. A method of producing a disease resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene that confers disease resistance.

20. A disease resistant stevia plant produced by the method of claim 19.

21. A method for developing a stevia plant in a stevia plant breeding program, comprising applying plant breeding techniques comprising marker enhanced selection, haploid/double haploid production, or transformation to the stevia plant of claim 1, or its parts, wherein application of said techniques results in development of a stevia plant.

23. A plant part consisting of pollen or embryos produced by growing the plant of claim 1, wherein said pollen and embryos comprise the single nucleotide polymorphism of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7 and SEQ ID NO;

8.

24. The tissue or cell culture of claim 5, produced from tissues or cells from a plant part selected from the group consisting of pollen and embryos, wherein said pollen and embryos comprise the single nucleotide polymorphism of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7 and SEQ ID NO;

8.

22. A Stevia rebaudiana seed, plant, plant part, or cell produced by crossing a plant or plant part of Stevia rebaudiana cultivar ‘

814011’

, or a locus conversion thereof, with another Stevia rebaudiana plant, wherein representative live plant tissue of said Stevia rebaudiana cultivar ‘

814011’

has been deposited under CGMCC No. 9701 and wherein said Stevia rebaudiana seed, plant, plant part, or cell produced from said cross has the same polymorphisms for the single nucleotide polymorphisms of SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7and SEQ ID NO;

8 as the plant or plant part of Stevia rebaudiana cultivar ‘

814011’

.