Methods for detecting modification resistant nucleic acids
First Claim
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1. A method for determining a ribosome footprint density, comprising:
- (a) isolating a plurality of monosomes from a plurality of polysomes, wherein each of said plurality of polysomes comprises a ribosome bound to a portion of a translatable RNA molecule;
(b) sequencing each of said portions; and
(c) determining a ribosome footprint density for each translatable RNA molecule from said sequencing, thereby determining a ribosome footprint density.
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Abstract
Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.
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12 Claims
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1. A method for determining a ribosome footprint density, comprising:
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(a) isolating a plurality of monosomes from a plurality of polysomes, wherein each of said plurality of polysomes comprises a ribosome bound to a portion of a translatable RNA molecule; (b) sequencing each of said portions; and (c) determining a ribosome footprint density for each translatable RNA molecule from said sequencing, thereby determining a ribosome footprint density. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification