Microfabrication methods for the optimal patterning of substrates
First Claim
1. A method of fabricating a microarray, comprising:
- (a) functionalizing a substrate having wells to introduce chemical modifications within said wells and surrounding said wells, the functionalizing involving;
reacting a reagent, selected from the group consisting of trichloro alkyl silane and trichlorosilane, with said substrate to create a modified substrate surface; and
attaching primer nucleic acids to the modified substrate surface, wherein the primer nucleic acids are complementary to a target nucleic acid;
wherein the chemical modifications include the modified substrate surface and the primer nucleic acids attached thereto;
(b) polishing, using mechanical treatment, said substrate to produce a polished surface surrounding said wells, whereby said chemical modifications surrounding said wells are removed and said chemical modifications within said wells are retained; and
(c) depositing said target nucleic acid onto said substrate, wherein higher affinity of said chemical modifications within said wells, compared to said polished surface, to said target nucleic acid facilitates localization of said target nucleic acid within said wells, wherein said affinity comprises complementarity of said primer nucleic acids to said target nucleic acid.
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Abstract
A method of fabricating a microarray including the steps of: (a) contacting a substrate having wells with a reagent reactive with said substrate to produce a surface modification within said wells and a surface modification surrounding said wells; (b) polishing said substrate to produce a polished surface modification surrounding said wells, wherein said surface modification surrounding said wells is removed and said surface modification within said wells is retained, and (c) depositing a biopolymer onto said substrate, wherein different affinities of said surface modification within said wells and said polished surface facilitate localization of said biopolymer within said wells.
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Citations
15 Claims
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1. A method of fabricating a microarray, comprising:
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(a) functionalizing a substrate having wells to introduce chemical modifications within said wells and surrounding said wells, the functionalizing involving; reacting a reagent, selected from the group consisting of trichloro alkyl silane and trichlorosilane, with said substrate to create a modified substrate surface; and attaching primer nucleic acids to the modified substrate surface, wherein the primer nucleic acids are complementary to a target nucleic acid; wherein the chemical modifications include the modified substrate surface and the primer nucleic acids attached thereto; (b) polishing, using mechanical treatment, said substrate to produce a polished surface surrounding said wells, whereby said chemical modifications surrounding said wells are removed and said chemical modifications within said wells are retained; and (c) depositing said target nucleic acid onto said substrate, wherein higher affinity of said chemical modifications within said wells, compared to said polished surface, to said target nucleic acid facilitates localization of said target nucleic acid within said wells, wherein said affinity comprises complementarity of said primer nucleic acids to said target nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of fabricating a microarray, comprising:
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(a) treating a substrate having wells to form a modified substrate surface within said wells and surrounding said wells, the treating involving reacting a reagent, selected from the group consisting of trichloro alkyl silane and trichlorosilane, with said substrate; (b) polishing, using mechanical treatment, said substrate to produce a polished surface surrounding said wells, whereby said modified substrate surface surrounding said wells is removed and said modified substrate surface within said well is retained; (c) attaching primer nucleic acids to said modified substrate surface within said wells, wherein the primer nucleic acids are complementary to a target nucleic acid; and (d) depositing said target nucleic acid onto said substrate, wherein an affinity of said primer nucleic acids within said wells to said target nucleic acid is higher than an affinity of said polished surface to said target nucleic acid, and wherein the higher affinity facilitates localization of said target nucleic acid within said wells.
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Specification