System and method for generating a wide-field OCT image of a portion of a sample
First Claim
Patent Images
1. A method for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the method comprises:
- creating a surface map of the sample;
acquiring raw OCT data of the portion of the sample based on the surface map by identifying an initial scan position and a final scan position on the surface map, the initial scan position corresponding to a highest point of the surface of the portion of the sample and the final scan position corresponding to a lowest point of the surface of the portion of the sample, and for a given set of vertical OCT images, acquiring the raw OCT data to generate at least a first OCT image with an upper edge of an imaging window aligned with the initial scan position and a second OCT image with an upper edge of an imaging window aligned with the final scan position;
generating a plurality of OCT images from the raw OCT data; and
combining two or more OCT images of the plurality of OCT images to create the wide field OCT image.
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Abstract
Various embodiments of systems and methods are described herein for obtaining wide field OCT images and other types of image data from at least one portion of a sample. Various embodiments of systems, and methods are described herein for assessing a degree of differentiation for a second region of an OCT image of a tissue sample with respect to another first. Various embodiments of a sample container are also described herein for containing a tissue sample and maintaining the tissue sample in a defined orientation during imaging or handling.
361 Citations
32 Claims
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1. A method for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the method comprises:
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creating a surface map of the sample; acquiring raw OCT data of the portion of the sample based on the surface map by identifying an initial scan position and a final scan position on the surface map, the initial scan position corresponding to a highest point of the surface of the portion of the sample and the final scan position corresponding to a lowest point of the surface of the portion of the sample, and for a given set of vertical OCT images, acquiring the raw OCT data to generate at least a first OCT image with an upper edge of an imaging window aligned with the initial scan position and a second OCT image with an upper edge of an imaging window aligned with the final scan position; generating a plurality of OCT images from the raw OCT data; and combining two or more OCT images of the plurality of OCT images to create the wide field OCT image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the system comprises:
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an input port for receiving raw OCT data; an image processing module being configured to process the raw OCT data to generate wide field OCT images by creating a surface map of the sample;
acquiring raw OCT data of the portion of the sample based on the surface map by identifying an initial scan position and a final scan position on the surface map, the initial scan position corresponding to a highest point of the surface of the portion of the sample and the final scan position corresponding to a lowest point of the surface of the portion of the sample, and for a given set of vertical OCT images, acquiring the raw OCT data to generate at least a first OCT image with an upper edge of an imaging window aligned with the initial scan position and a second OCT image with an upper edge of an imaging window aligned with the final scan position;
generating a plurality of OCT images from the raw OCT data; and
combining two or more OCT images of the plurality of OCT images to create the wide field OCT image; andan output port to provide the wide-field OCT images to one of a user, a storage device and another computing device. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A non-transitory computer-readable medium storing computer-executable instructions, the instructions for causing an image processing module to perform a method for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the method comprises:
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creating a surface map of the sample; acquiring raw OCT data of the portion of the sample based on the surface map by identifying an initial scan position and a final scan position on the surface map, the initial scan position corresponding to a highest point of the surface of the portion of the sample and the final scan position corresponding to a lowest point of the surface of the portion of the sample and for a given set of vertical OCT images, acquiring the raw OCT data to generate at least a first OCT image with an upper edge of an imaging window aligned with the initial scan position and a second OCT image with an upper edge of an imaging window aligned with the final scan position; generating a plurality of OCT images from the raw OCT data; and combining two or more OCT images of the plurality of OCT images to create the wide field OCT image.
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27. A method for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the method comprises:
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receiving a set of OCT images corresponding to the portion of the sample, the set of OCT images including a first OCT image and a neighbouring OCT image; aligning the first OCT image with the neighbouring OCT image; and overlaying a portion of the first OCT image over a portion of the neighbouring OCT image to create the wide field OCT image by (1) creating a plurality of intermediary composite images based on the first OCT image and the neighbouring image, each intermediary composite image having a different amount of overlap between the OCT image and the neighbouring image, (2) determining an entropy associated with the overlap in each intermediary composite image; and
(3) selecting the intermediary composite image associated with a lowest entropy value as the wide field OCT image for the first OCT image and the neighbouring OCT image and recording the overlap. - View Dependent Claims (28, 29, 30)
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31. A method for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the method comprises:
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creating a surface map of the sample; acquiring raw OCT data of the portion of the sample based on the surface map; generating a plurality of OCT images from the raw OCT data; combining two or more OCT images of the plurality of OCT images to create the wide field OCT image by; aligning a first OCT image with a neighbouring OCT image; creating a plurality of intermediary composite images by overlaying a portion of the first OCT image over a portion of the neighbouring image, wherein each intermediary composite image includes a different amount of overlap between the OCT image and the neighbouring image; determining an entropy associated with each intermediary composite image; and selecting the intermediary composite image associated with a lowest entropy value as the composite OCT image for the first OCT image and the neighbouring OCT image and recording the amount of overlap associated with the selected intermediary composite image.
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32. A system for generating a wide field optical coherence tomography (OCT) image of a portion of a sample, wherein the system comprises:
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an input port for receiving raw OCT data; an image processing module being configured to process the raw OCT data to generate wide field OCT images by creating a surface map of the sample;
acquire raw OCT data of the portion of the sample based on the surface map;
generate a plurality of OCT images from the raw OCT data; and
combine two or more OCT images of the plurality of OCT images to create the wide field OCT image by;aligning the first OCT image with a neighbouring OCT image; overlaying a portion of the first OCT image over a portion of the neighbouring OCT image to create a composite OCT image; creating a plurality of intermediary composite images by overlaying a portion of the first OCT image over a portion of the neighbouring image, wherein each intermediary composite image includes a different amount of overlap between the OCT image and the neighbouring image; determining an entropy associated with each intermediary composite image; and selecting the intermediary composite image associated with a lowest entropy value as the composite OCT image for the first OCT image and the neighbouring OCT image and recording the amount of overlap associated with the selected intermediary composite image; and an output port to provide the wide-field OCT images to one of a user, a storage device and another computing device.
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Specification