Complex support body for regenerating bone-cartilage, method for manufacturing thereof, and composition for treating bone and cartilage related diseases comprising same as active ingredient

• US 9,681,953 B2
• Filed: 02/20/2012
• Issued: 06/20/2017
• Est. Priority Date: 03/31/2011
• Status: Active Grant

• Current Assignee: Industry-Academia Cooperation Group (Inje University)
• Original Assignee: Industry-Academia Cooperation Group (Inje University)
• Inventors: Shin, Jung Woog, Shin, Ji Won, Park, So Hee
• Primary Examiner(s): Willse, David H
• Assistant Examiner(s): BLANCO, JAVIER G
• Application Number:
  US14/006,401
• Publication Number:
  US 20140012393A1
• Time in Patent Office: 1,947 Days
• Field of Search: None
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61F 2/3094 Designing or manufacturing processes
• CPC Class Codes: A61F 2002/30766 Scaffolds for cartilage ingrowth and regeneration
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/12 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61L 27/3817 Cartilage-forming cells, e.g. pre-chondrocytes
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body

Abstract

The present invention relates to a complex support body for regenerating bone-cartilage, a method for manufacturing thereof, and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient, and more particularly, to a complex support body for regenerating bone-cartilage, which comprises a bone regeneration layer consisting of a biodegradable polymer and a biocompatible ceramic, and a cartilage regeneration layer, which is coupled to the bone regeneration layer and in which cells that can be differentiated into cartilages cells are fixed; a method for manufacturing thereof; and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient. The complex support body of the present invention for regenerating bone-cartilage is manufactured as a three-dimensional support body, which is similar to a living tissue, according to a bioplotting method, and exerts the effect of regeneration into a bone tissue and a cartilage tissue, respectively, depending on materials encountered in the environment where the complex support body for regenerating bone-cartilage is used.

Claims

1. A bone-cartilage regeneration support body for regenerating bone-cartilage, the support body comprising:

(a) a bone regeneration layer, in which cells that can be differentiated into bones cells are fixed; and

(b) a discrete cartilage regeneration layer, which is coupled to the bone regeneration layer and in which cells that can be differentiated into cartilage cells are fixed,

wherein;

the bone regeneration layer comprises a biodegradable polymer in an amount of 85 to 95 wt % and a biocompatible ceramic in an amount of 5 to 15 wt %;

the cartilage regeneration layer is coupled to the bone regeneration layer with at least one biocompatible glue selected from the group consisting of fibrin glue, gelatin glue, polyurethane-containing glue, and cyanoacrylate-containing glue;

the bone regeneration layer comprises pores in the size of 100 μ

m to 700 μ

m, and the cartilage regeneration layer comprises pores in the size of 50 μ

m to 700 μ

m;

the biodegradable polymer is poly-caprolactone (PCL);

the biocompatible ceramic is hydroxyapatite (HA);

the cartilage regeneration layer is a cell-fixed biodegradable polymer support body consisting of alginate; and

the bone regeneration layer and the cartilage regeneration layer are manufactured separately by a bio-plotting technique of a rapid prototype method, and then securely coupled to each other.

2. The complex support body for regenerating bone-cartilage according to claim 1, wherein the cells that can be differentiated into cartilage cells is at least one cells selected from the group consisting of:

mesenchymal stem cells and interstitial cells, which are isolated from any one of bone marrow, muscle, fat, umbilical cord, amnion and amniotic fluid; and

precursor cells, which are derived from the cells and can be differentiated into cartilage cells.