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Tip overlay for continuous flow spotting apparatus

  • US 9,682,372 B2
  • Filed: 06/19/2009
  • Issued: 06/20/2017
  • Est. Priority Date: 06/19/2008
  • Status: Active Grant
First Claim
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1. A spotter apparatus for depositing a substance from a carrier fluid onto a deposition surface in the form of a deposition spot, the spotter apparatus, comprising:

  • a) a loading surface including a first well and a second well;

    b) an outlet surface, different than the loading surface, including a first opening and a second opening;

    c) a first microconduit fluidly coupling the first well to the first opening;

    d) a second microconduit fluidly coupling the second well to the second opening; and

    e) a tip overlay positioned on the outlet surface and penetrated by a deposition channel, wherein the deposition channel is situated to communicate carrier fluid among the first opening, the second opening, and a deposition surface when the tip overlay is pressed against the deposition surface,wherein the spotter apparatus is in the form of an array for applying multiple deposition spots on the deposition surface;

    wherein the array is operable for applying individual deposition spots by their own unique first well, second well, first microconduit, second microconduit, first opening, second opening, and deposition channel;

    wherein the loading surface, the outlet surface, the first microconduit and the second microconduit are formed in multiple layers substantially parallel to the loading surface;

    wherein the tip overlay is substantially orthogonal to the multiple layers; and

    wherein a first deposition channel is aligned with one of the multiple layers and a second deposition channel is aligned with another of the multiple layers.

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