Methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries
First Claim
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1. A vector comprising:
- (i) a DNA sequence encoding an antibody variable region linked to a fragment of a wild-type pIII anchor, which does not mediate infection of phage particles; and
(ii) a wild-type gene III;
wherein the nucleic acid sequence encoding the fragment of the wild-type pIII anchor in (i) differs from the portion of the wild-type gene III encoding the same fragment to an extent sufficient to reduce homologous recombination between the nucleic acid encoding the fragment of the wild-type PIII anchor in (i) and the wild-type gene III in (ii).
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Abstract
Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.
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20 Claims
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1. A vector comprising:
- (i) a DNA sequence encoding an antibody variable region linked to a fragment of a wild-type pIII anchor, which does not mediate infection of phage particles; and
(ii) a wild-type gene III;
wherein the nucleic acid sequence encoding the fragment of the wild-type pIII anchor in (i) differs from the portion of the wild-type gene III encoding the same fragment to an extent sufficient to reduce homologous recombination between the nucleic acid encoding the fragment of the wild-type PIII anchor in (i) and the wild-type gene III in (ii). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- (i) a DNA sequence encoding an antibody variable region linked to a fragment of a wild-type pIII anchor, which does not mediate infection of phage particles; and
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