Device for receiving small volume liquid samples

• US 9,683,927 B2
• Filed: 11/08/2012
• Issued: 06/20/2017
• Est. Priority Date: 12/02/2011
• Status: Active Grant

• Current Assignee: Biochrom Limited (Harvard Bioscience, Inc.)
• Original Assignee: Biochrom Limited (Harvard Bioscience, Inc.)
• Inventors: Gifford, Graham Eric
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US14/362,206
• Publication Number:
  US 20140356234A1
• Time in Patent Office: 1,685 Days
• Field of Search: None
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01N 2021/035 Supports for sample drops
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 2201/0846 Fibre interface with sample, e.g. for spatial resolution

Abstract

Improvements in and relating to devices for receiving liquid samples A device for receiving a liquid sample may form part of a micro sampling head for an instrument such as a spectrophotometer. The device receives a liquid sample to be analyzed by a process involving the passing of electromagnetic radiation through the sample, and comprises a light inlet guide (20) for directing electromagnetic radiation into the sample, a light receiving element (23) situated in an opposed relationship to the guide and spaced from the guide by a fixed distance to define a fixed path length gap (21), which is, in use, filled with the sample. In use, radiation is passed from the light inlet guide to the light receiving element (23), and the path length of radiation through the sample is defined by the gap (23). The device is open or openable to allow a droplet of sample to be deposited directly in the gap.

Claims

1. A device for receiving a liquid sample to be analysed by a process of involving the passing of electromagnetic radiation through the sample, the device comprising a housing assembly, a light inlet guide supported by the housing assembly for directing electromagnetic radiation into the sample, a light receiving element supported by the housing assembly, situated in an opposed relationship to the guide, and spaced from the guide by a fixed distance to define a fixed length gap for receiving, and being filled with, the sample, the device being operable to cause the radiation to pass from the light inlet guide to the light receiving element, the path length of radiation through the sample being defined by said gap, the housing assembly having a recess to allow a droplet of the sample to be deposited directly in the gap, wherein the gap is situated in the recess;

wherein the light inlet guide is elongate, having an output end face positioned within the recess and through which, in use, electromagnetic radiation is emitted into said gap;

wherein the light inlet guide comprises an inlet optical fibre including first and second regions, wherein the first region consists of a fibre core surrounded by a flexible cladding, and the second region consists of a rigid metallic cladding encasing the fibre core, and wherein the second region is located coincident with the output end face and adjacent to the gap.

2. The device according to claim 1, in which the output end face constitutes one side of the gap, the light receiving element constituting the other side.

3. The device according to claim 1, in which the position of the output end face relative to the light receiving element is fixed.

4. The device according to claim 1, in which the axis of the inlet optical fibre, at least at said output end face, intersects said light receiving element.

5. The device according to claim 1, in which the rigid metallic cladding extends to the end of the inlet optical fibre at which said output end face is situated.

6. The device according to claim 1, in which the rigid metallic cladding is bonded to the core of the inlet optical fibre.

7. The device according to claim 1, in which the rigid metallic cladding consists of a tube.

8. The device according to claim 7, in which the tube is a steel tube.

9. The device according to claim 8, in which the core directly bears against the steel tube.

10. The device according to claim 1, in which the light receiving element comprises a receiving end face of a further optical fibre, the output end face and the receiving end face thus defining the gap.

11. The device according to claim 10, in which the further optical fibre is of the same construction as the inlet optical fibre, said further optical fibre including third and fourth regions, wherein the third region consists of a further fibre core surrounded by a further flexible cladding, and the fourth region consists of a further rigid metallic cladding encasing the further fibre core, and wherein the fourth region is located coincident with the receiving end face and adjacent to the gap.

12. The device according to claim 11, in which the inlet optical fibre and the further optical fibre are co-axial, at least in the region of the receiving end face and the output end face.

13. The device according to claim 12, in which the coaxial portions of the inlet optical fibre and the further optical fibre are horizontal, so that each of the receiving end face and the output end face defines a respective side of the gap.

14. The device according to claim 11, in which the receiving end face and the output end face and the rigid metallic claddings of the inlet optical fibre and the further optical fibre stand proud of the recess.

15. The device according to claim 11, in which the inlet optical fibre and the further optical fibre are the only optical fibres extending into the recess.

16. The device according claim 10, in which the gap is so sized and positioned that a single 1 microliter drop of liquid will fill or flood the gap.

17. The device according to claim 16, in which the length of the gap is 0.5 millimeters plus or minus 1%.

18. A spectrophotometer comprising a device for receiving a liquid sample to be analysed by the spectrophotometer, the device comprising a housing assembly, a light inlet guide supported by the housing assembly for directing electromagnetic radiation into the sample, the light inlet guide having an inlet, and a light receiving element supported by the housing assembly, situated in an opposed relationship to the guide, and spaced from the guide by a fixed distance to define a fixed length gap for receiving, and being filled with, the sample, the spectrophotometer further comprising an electromagnetic radiation source for emitting electromagnetic radiation which is incident on an inlet end of the light inlet guide, the device being operable to cause the radiation to pass from the light inlet guide to the light receiving element, a path length of radiation through the sample being defined by said gap, the spectrophotometer further comprising a monochromator for analysing the radiation received by the light receiving element, wherein the housing assembly has a recess to allow a droplet of the sample to be deposited directly in the gap, wherein the gap is situated in the recess;

wherein the light inlet guide is elongate, having an output end face positioned within the recess and through which, in use, electromagnetic radiation is emitted into said gap;

wherein the light inlet guide comprises an inlet optical fibre including first and second regions, wherein the first region consists of a fibre core surrounded by a flexible cladding, and the second region consists of a rigid metallic cladding encasing the fibre core, and wherein the second region is located coincident with the output end face and adjacent to the gap.