Robust colorimetric processing method for paper based sensors
First Claim
Patent Images
1. A colorimetric processing method for paper based sensors, comprising:
- using a camera and taking a picture of a colorimetric paper sensor for colorimetric reactions;
identifying the type of paper sensor by image processing;
identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption;
identifying the known absorption ratio between the dye and the reference color in each color channel;
separating each test area in the channel from the non-test areas;
normalizing each absorption channel by the near zero absorption channel to remove spatial variation;
calibrating the test area reading with the reference color and a substrate white in the composite image; and
,reporting the concentration of a test substance analyte based on the calibrated reading in each test area.
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Abstract
The present disclosure proposes a colorimetric method that couples sensor design with image processing to enable automated evaluation of test results obtained by paper-based sensors. The proposed method can match ink color and dye used in colorimetric reaction in terms of their absorption in spectral range (e.g., red, green, blue). A near-zero absorption channel can then be used to normalize absorption channels and construct a composite image.
58 Citations
21 Claims
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1. A colorimetric processing method for paper based sensors, comprising:
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using a camera and taking a picture of a colorimetric paper sensor for colorimetric reactions; identifying the type of paper sensor by image processing; identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption; identifying the known absorption ratio between the dye and the reference color in each color channel; separating each test area in the channel from the non-test areas; normalizing each absorption channel by the near zero absorption channel to remove spatial variation; calibrating the test area reading with the reference color and a substrate white in the composite image; and
,reporting the concentration of a test substance analyte based on the calibrated reading in each test area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A colorimetric processing method for paper based sensors, comprising:
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taking a picture of a colorimetric paper sensor for colorimetric reactions; identifying the type of paper sensor by image processing; identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption; identifying the known absorption ratio between the dye and the reference color in each color channel; separating each test area in the channel from the non-test areas; normalizing each absorption channel by the near zero absorption channel to remove spatial variation; wherein the colorimetric paper sensor is a biomedical paper sensor; wherein the paper sensor includes a plurality of axially radiating test zones; each of the axially radiating test zones divided by wax ink barrier walls; each of the axially radiating test zones contain a unique test reagent therein; a reference region surrounding the plurality of axially radiating test zones; and
,wherein the reference region includes a calibration color area including a predeterminable color for comparing to one or more of the axially radiating test zones. - View Dependent Claims (12, 13)
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14. A colorimetric processing method for paper based sensors, comprising:
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taking a picture of a colorimetric paper sensor for colorimetric reactions; identifying the type of paper sensor by image processing; identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption; identifying the known absorption ratio between the dye and the reference color in each color channel; separating each test area in the channel from the non-test areas; normalizing each absorption channel by the near zero absorption channel to remove spatial variation; wherein the colorimetric paper sensor is a biomedical paper sensor; wherein the paper sensor includes a plurality of axially radiating test zones; each of the axially radiating test zones divided by wax ink barrier walls; each of the axially radiating test zones contain a unique test reagent therein; a reference region surrounding the plurality of axially radiating test zones; a total device area including the combined areas of a reference region area and the axially radiating test zones area; wherein the axially radiating test zones area is at least 37.5% of the total device area; and
,wherein the reference region area further includes a substrate region separating the axially radiating test zones area and the calibration color area. - View Dependent Claims (15)
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16. A colorimetric processing method for paper based sensors, comprising:
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taking a picture of a colorimetric paper sensor for colorimetric reactions; identifying the type of paper sensor by image processing; identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption; identifying the known absorption ratio between the dye and the reference color in each color channel; separating each test area in the channel from the non-test areas; normalizing each absorption channel by the near zero absorption channel to remove spatial variation; and
,wherein the colorimetric paper sensor is a biomedical paper sensor. - View Dependent Claims (17, 18, 19, 20, 21)
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Specification