×

Methods and systems for sequencing long nucleic acids

  • US 9,689,032 B2
  • Filed: 08/19/2013
  • Issued: 06/27/2017
  • Est. Priority Date: 04/01/2011
  • Status: Active Grant
First Claim
Patent Images

1. A method for sequencing a target nucleic acid, comprising:

  • (a) sequencing one or more bases of a target nucleic acid by extending a first sequencing primer hybridized to said target nucleic acid to generate a first primer extension product, thereby obtaining a first sequence read;

    (b) releasing said first primer extension product from said target nucleic acid;

    (c) hybridizing a second sequencing primer to said target nucleic acid;

    (d) generating a second primer extension product by extending said second sequencing primer through limited extension without the use of labeled nucleotides;

    (e) sequencing one or more bases of said target nucleic acid by further extending said second primer extension product to generate a third primer extension product, thereby obtaining a second sequence read; and

    (f) determining a sequence of said target nucleic acid by assembling said first sequence read and said second sequence read.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×