Use of glycolytic pathways for inhibiting or measuring oncogenic signaling
First Claim
1. A method, comprising:
- measuring an expression level of at least one cellular marker selected from the group consisting of;
platelet-type phosphofructokinase (PFKP);
glyceraldehyde-3-phosphate dehydrogenase (GAPDH);
aldolase (ALDO);
lactate dehydrogenase A (LDHA);
glucose transporter 3 (GLUT3); and
glutamine-fructose-6-phosphate transaminase (GFPT), in a cell suspected of having increased oncogenic signaling; and
determining whether the measured expression level of said at least one cellular marker in said cell suspected of having increased oncogenic signaling is increased relative to the expression level of said at least one cellular marker in a reference cell.
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed are methods in which glucose metabolism is correlated to oncogenesis through certain specific pathways; inhibition of certain enzymes is shown to interfere with oncogenic signaling, and measurement of certain enzyme levels is correlated with patient survival. The present methods comprise measuring level of expression of at least one of the enzymes involved in glucose uptake or metabolism, wherein increased expression of the at least one of the enzymes relative to expression in a normal cell correlates with poor prognosis of disease in a patient. Preferably the genes whose expression level is measured include GLUT3, PFKP, GAPDH, ALDOC, LDHA and GFPT2. Also disclosed are embodiments directed towards downregulating the expression of some genes in glucose uptake and metabolism.
6 Citations
15 Claims
-
1. A method, comprising:
-
measuring an expression level of at least one cellular marker selected from the group consisting of;
platelet-type phosphofructokinase (PFKP);
glyceraldehyde-3-phosphate dehydrogenase (GAPDH);
aldolase (ALDO);
lactate dehydrogenase A (LDHA);
glucose transporter 3 (GLUT3); and
glutamine-fructose-6-phosphate transaminase (GFPT), in a cell suspected of having increased oncogenic signaling; anddetermining whether the measured expression level of said at least one cellular marker in said cell suspected of having increased oncogenic signaling is increased relative to the expression level of said at least one cellular marker in a reference cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
-
Specification