Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
First Claim
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1. A nucleic acid probe set consisting of:
- a) a first nucleic acid probe consisting of one of the nucleotide sequences selected from the group consisting of SEQ ID NO;
102 to SEQ ID NO;
113, and complements thereof and a detectable label, wherein said first nucleic acid probe is capable of hybridizing under selective stringency conditions to a portion of a gene selected from the group consisting of E6 and E7; and
b) a second nucleic acid probe consisting of one of the nucleotide sequences selected from the group consisting of SEQ ID NO;
577 to SEQ ID NO;
580, wherein said second nucleic acid probe is capable of hybridizing under selective stringency conditions to a portion of an L1 gene;
wherein the first nucleic acid probe and second nucleic acid probe are capable of hybridizing under stringent conditions to the same HPV genome, andwherein the nucleic acid probe set is capable of isolating HPV nucleic acids from non-HPV nucleic acids in a sample.
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Abstract
Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step using two separate sequence-specific polynucleotide probes. Also provided are nucleic acids comprising SEQ ID NO: 1 to SEQ ID NO: 727 and nucleic acid probes and probe sets comprising the same.
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Citations
7 Claims
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1. A nucleic acid probe set consisting of:
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a) a first nucleic acid probe consisting of one of the nucleotide sequences selected from the group consisting of SEQ ID NO;
102 to SEQ ID NO;
113, and complements thereof and a detectable label, wherein said first nucleic acid probe is capable of hybridizing under selective stringency conditions to a portion of a gene selected from the group consisting of E6 and E7; andb) a second nucleic acid probe consisting of one of the nucleotide sequences selected from the group consisting of SEQ ID NO;
577 to SEQ ID NO;
580, wherein said second nucleic acid probe is capable of hybridizing under selective stringency conditions to a portion of an L1 gene;wherein the first nucleic acid probe and second nucleic acid probe are capable of hybridizing under stringent conditions to the same HPV genome, and wherein the nucleic acid probe set is capable of isolating HPV nucleic acids from non-HPV nucleic acids in a sample. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification