Cancer cell detection using dielectrophoretic dynamic light scattering (DDLS) spectroscopy

• US 9,693,694 B2
• Filed: 09/23/2014
• Issued: 07/04/2017
• Est. Priority Date: 11/21/2012
• Status: Active Grant

• Current Assignee: Folim G. Halaka
• Original Assignee: Folim G. Halaka
• Inventors: Halaka, Folim G.
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Fardanesh, Marjan
• Application Number:
  US14/494,565
• Publication Number:
  US 20150133794A1
• Time in Patent Office: 1,015 Days
• Field of Search: None
• CPC Class Codes: A61B 2562/0233 Special features of optical sensors or probes classified in A61B5/00
• CPC Class Codes: A61B 5/0093 Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
• CPC Class Codes: A61B 5/0507 using microwaves or terahertz waves
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/7257 using Fourier transforms
• CPC Class Codes: B03C 2201/26 for use in medical or biological applications
• CPC Class Codes: B03C 5/005 Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 2015/0222 from dynamic light scattering, e.g. photon correlation spectroscopy

Abstract

Non-invasive apparatus and method for determining and monitoring glucose concentrations in human subjects. Glucose level is estimated through the effect of glucose on biological cells with glucose dependencies, e.g., red blood cells. The invention is based on the interaction of such cells with oscillating electric field gradients. The response of biological cells depends on factors including shape, size, and electrical charge distribution. The field gradient causes the cells to undergo characteristic motion which is detected by light beam scattering. The autocorrelation of the scattered light is computed, and the Fourier transform (FT) is performed to produce a characteristic velocity spectrum in which the peaks are characteristic of the cell “bio-electrical” states. The glucose level is estimated through measurements of changes of FT with changes in glucose levels after calibration with standard glucose methods.

Claims

1. A non-invasive apparatus for detection of cancer cells in a biological sample, said apparatus comprising:

an elongated housing having a distal end and a proximal end;

a pair of electrodes mounted at the distal end of the housing, the electrodes comprising an arcuate first electrode and a linear second electrode substantially centered in the arcuate first electrode;

an oscillating power source constructed to produce a sinusoidal electric voltage;

the oscillating power source electrically connected to said electrodes through a cable from the oscillating power source, through the proximal end of the housing to the electrodes;

wherein said electrodes generate a non-uniform sinusoidal electric field between the arcuate and linear electrodes;

a light source attached to the distal end of the housing constructed to deliver collimated light to a biological sample being exposed to said non-uniform electric field producing scattered light therefrom;

a detector also attached to the distal end of the housing adapted to collect the scattered light;

a processor mounted in said housing, the processor processing electrical signals from the detector related to the scattered light to produce an autocorrelation output signal, the processor comparing the autocorrelation output signal with stored autocorrelation signals taken from biological samples containing known cancer cells.

2. The non-invasive apparatus of claim 1 wherein said light source is a laser, and wherein said detector is a photomultiplier or a photo-diode.

3. The non-invasive apparatus of claim 1 wherein said oscillating electrical power source supplies an oscillating voltage of between approximately 1 volt and 1000 volts, and a frequency between approximately 0 Hz and 100 GHz.