Peptide domains that bind small molecules

• US 9,695,217 B2
• Filed: 12/14/2015
• Issued: 07/04/2017
• Est. Priority Date: 01/29/2010
• Status: Active Grant

• Current Assignee: Archer Daniels Midland Company, Iowa State University Research Foundation Incorporated (Iowa State University)
• Original Assignee: Archer Daniels Midland Company, Iowa State University Research Foundation Incorporated (Iowa State University)
• Inventors: Binder, Thomas P., Rao, Aragula Gururaj, Yamamoto, Yasufumi, Hanke, Paul D.
• Primary Examiner(s): Steele, Amber D
• Assistant Examiner(s): Milton, Schuyler
• Application Number:
  US14/968,386
• Publication Number:
  US 20160376310A1
• Time in Patent Office: 568 Days
• Field of Search: None
• CPC Class Codes: C07K 14/00 Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
• CPC Class Codes: C07K 5/08 Tripeptides
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68

Abstract

Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

Claims

1. An isolated non-naturally occurring fusion polypeptide or isolated non-naturally occurring fusion peptide comprising a carotenoid binding peptide comprising a sequence of the formula

X¹X²GWX³HyX⁴X⁵X⁶ 

 

(SEQ ID NO;

120)

Wherein Hy is an aromatic amino acid;

X¹ is alanine, valine, leucine, glutamine, tryptophan, tyrosine, serine, proline, threonine or isoleucine;

X² is alanine, glycine, isoleucine or valine;

X³ is tryptophan, methionine, glycine, proline, leucine, or serine;

X⁴ is tryptophan, phenylalanine, methionine, glycine, threonine, or histidine;

X⁵ is glycine, tryptophan, serine, phenylalanine, leucine, glutamine, or alanine; and

X⁶ is threonine, glycine, tryptophan, alanine, methionine, asparagine, or valine.

2. The isolated polypeptide or peptide of claim 1 wherein X² is alanine.

3. The isolated polypeptide or peptide of claim 1 wherein Hy is tryptophan.

4. The isolated polypeptide or peptide of claim 1 wherein X⁴ is a tryptophan.

5. An isolated non-naturally occurring fusion polypeptide or isolated non-naturally occurring fusion peptide comprising a carotenoid binding peptide comprising a sequence

X¹WX²Hy 

 

(SEQ ID NO;

121)

where Hy is an aromatic amino acid;

X² is any amino acid; and

X¹ is a single amino acid selected from glycine, proline or leucine.

6. The isolated polypeptide or peptide of claim 5 wherein X¹ is glycine.