Regenerated tissue comprising a stratified structure of epithelial cells

• US 9,695,396 B2
• Filed: 10/21/2013
• Issued: 07/04/2017
• Est. Priority Date: 10/15/2001
• Status: Expired due to Fees

• Current Assignee: Shigekazu Nakatsugawa
• Original Assignee: Shigekazu Nakatsugawa
• Inventors: Nakatsugawa, Shigekazu
• Primary Examiner(s): DUNSTON, JENNIFER ANN
• Application Number:
  US14/058,885
• Publication Number:
  US 20140147831A1
• Time in Patent Office: 1,352 Days
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/3813 Epithelial cells, e.g. keratinocytes, urothelial cells
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 2502/088 neural stem cells
• CPC Class Codes: C12N 2502/095 mammary cells
• CPC Class Codes: C12N 2502/246 Cells of the male genital tract, non-germinal testis cells
• CPC Class Codes: C12N 2502/256 Renal cells
• CPC Class Codes: C12N 2502/28 Vascular endothelial cells
• CPC Class Codes: C12N 2502/30 tumour cells
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2503/04 Screening or testing on artificial tissues
• CPC Class Codes: C12N 5/0625 Epidermal cells, skin cells; Cells of the oral mucosa
• CPC Class Codes: C12N 5/0697 Artificial constructs associating cells of different lineages, e.g. tissue equivalents blood vessels C12N5/0691

Abstract

A normal regenerated tissue is formed by exposing to radiation a connective tissue or a supporting tissue originating in an organ to thereby form a feeder layer and then transplanting epithelial cells thereon to form a stratified structure. By conveniently and surely providing regenerated tissue by the 3-dimensional culture with the use of a human-origin normal tissue as a base, it is possible to construct systems for assessing effects and side effects of chemicals such as drugs or assessing sensitivities thereof with the use of regenerated tissues as models of corresponding tissues respectively.

Claims

1. An isolated regenerated tissue comprising a stratified structure of epithelial cells on an irradiated feeder layer of fibroblast and vascular endothelial cells obtained from an organ, wherein the fibroblasts, the endothelial cells and the epithelial cells are orthotopic with regard to the originating organ, wherein the organ is not skin;

wherein the feeder layer is formed by irradiating the combination of the vascular endothelial cells and the fibroblast cells with 20 Gy or less X-ray or γ

-ray; and

wherein a ratio of the fibroblasts to the vascular endothelial cells is 10;

1.

2. The isolated regenerated tissue according to claim 1, wherein the epithelial cells are selected from the group consisting of oral mucosa epithelial cells, bronchus epithelial cells, mammary gland epithelial cells, liver epithelial cells, prostate epithelial cell, and kidney epithelial cells.

3. The isolated regenerated tissue according to claim 1, wherein the feeder layer consists of a lower layer of the fibroblasts and upper layer of the endothelial cells.

4. The isolated regenerated tissue according to claim 1, wherein the epithelial cells are in a stratified structure consisting of four or more layers.

5. The isolated regenerated tissue according to claim 1, wherein the regenerated tissue is on a basal plate.

6. The isolated regenerated tissue according to claim 1, wherein the regenerated tissue contacts a culture medium or culture fluid.

7. An in vitro organ model comprising the isolated regenerated tissue according to claim 1.