Methods for enhancing bacterial cell display of proteins and peptides
First Claim
1. A circularly permuted OmpX (CPX) variant comprising a linker joining the native N-terminus and native C-terminus of OmpX, wherein the CPX variant comprises a non-native N-terminus and a non-native C-terminus, wherein the linker is 3-8 residues in length and comprises a sequence GXnZm,wherein each X is independently any amino acid, each Z is independently threonine, lysine, arginine, glutamine, asparagine, or histidine, n is 0 to 4, and m is 1 to 4.
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Abstract
Methods of making and using bacterial display polypeptide libraries using circularly permuted OmpX (CPX) variants are disclosed. The invention further relates to methods for enhancing the display of proteins and peptides at the surface of bacteria by optimizing linkers and incorporating mutations at positions 165 and 166 of CPX.
45 Citations
42 Claims
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1. A circularly permuted OmpX (CPX) variant comprising a linker joining the native N-terminus and native C-terminus of OmpX, wherein the CPX variant comprises a non-native N-terminus and a non-native C-terminus, wherein the linker is 3-8 residues in length and comprises a sequence GXnZm,
wherein each X is independently any amino acid, each Z is independently threonine, lysine, arginine, glutamine, asparagine, or histidine, n is 0 to 4, and m is 1 to 4.
Specification