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Methods and apparatus for single molecule sequencing using energy transfer detection

  • US 9,695,471 B2
  • Filed: 12/29/2014
  • Issued: 07/04/2017
  • Est. Priority Date: 03/27/2009
  • Status: Active Grant
First Claim
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1. A method for generating an energy transfer signal comprising the steps of:

  • contacting (i) a polymerase having altered nucleotide incorporation kinetics and linked to an energy transfer donor moiety with (ii) a nucleic acid molecule and with (iii) at least one type of a nucleotide having an energy transfer acceptor moiety, so as to incorporate the nucleotide into the nucleic acid molecule thereby locating the polymerase and nucleotide in close proximity with each other to generate the energy transfer signal, wherein the polymerase comprises the amino acid sequence of SEQ ID NO;

    2.

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