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Methods for isolating a target analyte from a heterogeneous sample

  • US 9,696,302 B2
  • Filed: 09/05/2014
  • Issued: 07/04/2017
  • Est. Priority Date: 04/21/2010
  • Status: Active Grant
First Claim
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1. A method for isolating pathogen from a heterogeneous sample, the method comprising:

  • providing a vessel that contains a heterogeneous sample comprising pathogen, the vessel being coupled to a fluidic device that comprises a channel and a magnet;

    introducing to the sample in the vessel a cocktail comprising a plurality of magnetic particles, conjugated to antibodies that bind said pathogen, thereby forming a mixture comprising antibody-bound pathogen particles;

    flowing the mixture from the vessel through a channel in the fluidic device such that said magnetic particles attach to said magnet; and

    separating antibody-bound pathogen attached to said magnet from other components in the sample.

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